Expression Of SAPCD2 In Colorectal Cancer And The Role Of SAPCD2 In Cell Proliferation,Migration And Invasion Ability

Objective To investigate the expression of Suppressor Anaphase-promoting complex domain containing 2（SAPCD2）in colorectal carcinoma and explore the potential biological roles of SAPCD2 played in proliferation,migration and invasion of CRCs.Methods 1.Immunohistochemistry for SAPCD2 was performed in paraffin-embedded colorectal carcinoma specimens and corresponding normal epithelium tissues,and in adenoma tissues.Quantitative Real-Time PCR and Western blot were carried out to evaluate SAPCD2 expression in colorectal carcinoma samples and paracancerous relative normal epithelium tissues.2.The CRC cell lines HCT 116 and RKO were transfected with pLV-shRNA SAPCD2/pLV-shControl and lenti-OE SAPCD2/control,respectively.Biological functions of SAPCD2 in vitro on cell proliferation were evaluated by MTT,colony formation and Celigo cell counting assay,cell migration and invasion were analyzed by Transwell assay.Distribution of cell cycle was analyzed by PI-FACS assay.Biological function of SAPCD2 in vivo was investigated through nude mice assay.Results 1.Immunohistochemistry showed that SAPCD2 expression was significantly higher in colorectal carcinoma tissues compared with normal epithelium tissues and adenoma tissues（P<0.001）.The higher expression of SAPCD2 in colorectal carcinoma tissues was related with left tumor location（P=0.018）.The expression of SAPCD2 had no relationship with the gender,age,tumor size,tumor differentiation,TNM stage,depth of invasion and lymph node metastasis（P>0.05）.Quantitative Real-Time PCR and Western blot results showed that SAPCD2 expression in colorectal carcinoma tissues was significantly higher than paracancerous relative normal epithelium tissues at the protein and mRNA level（P<0.001,P=0.045）.2.SAPCD2 knockdown in RKO and HCT116 cells obviously inhibited cell proliferation,migration and invasion（P<0.05）,while SAPCD2overexpression in RKO cells significantly promoted cell proliferation and migration（P<0.05）in vitro.PI-FACS results showed that SAPCD2 knockdown in RKO and HCT116 cells was related with reduced G₁/S transition（P<0.05）,and SAPCD2overexpression was associated with G₂/M phase arrest（P<0.05）.Nude mice result showed that SAPCD2 knockdown in RKO dramatically decreased tumor formation ability in vivo（P<0.05）.Conclusions SAPCD2 is overexpressed in colorectal carcinoma tissues.SAPCD2overexpression affect distribution of cell cycle in CRC cell,and promote the proliferation,migration and invasion ability of CRC cells in vitro.SAPCD2 knockdown inhibit the tumor formation ability in vivo.