Exploring Serum Asprosin Levels And Its Influencing Factors In People With Different Glucose Tolerance

Objective: To monitor the changes of serum Asprosin levels in people with different glucose tolerances.And analyzed its correlation with glucose metabolism parameters,blood lipids,systemic fat content,visceral fat area,inflammatory index TNF-α,Further explore its role in the development of diabetes and its possible mechanisms.Methods:some people in Shijiazhuang City who were 18-70 years old were recruited During the period from September 2018 to June 2019.Informed consent was signed according to the voluntary principle to participate in the study,a total of 328 people were recruited,and all subjects were divided into three groups according to the results of oral glucose tolerance test(OGTT).There were 118 cases of normal glucose tolerance group(NGR),123 cases of impaired glucose regulation(IGR),and 87 cases of newly diagnosed type 2 diabetes mellitus(T2DM).Collect information such as age,gender,and past medical history.General measurement indicators such as height,weight,waist circumference,and hip circumference were measured,and body mass index(BMI)and waist-to-hip ratio(WHR)were calculated.Measurement of liver function,renal function,blood lipids,glycosylated hemoglobin,fasting blood glucose,Fasting insulin,2h blood glucose,2h insulin and other indicators,calculate insulin resistance index(HOMA-IR)and islet cell function index(HOMA-β).The serum Asprosin and TNF-α concentrations were measured by ELISA,and the percentage of whole body fat and visceral fat area(VAT)were measured by dual energy X-ray method.Result:1.Comparison of general measurement data and biochemical measurement results between the three groups.Comparison of general measurement data:There were no statistically significant differences in gender,age,past medical history,family history,systolic blood pressure,and diastolic blood pressure between the three groups.The BMI,waist circumference,and waist-to-hip ratio were statistically different between the three groups(P=0.045,P=0.034,P=0.038,respectively).Compared with the NGR group,the BMI,waist circumference,waist-to-hip ratio of the IGR group and the T2 DM group were significantly increased(all P<0.05).Compared with the IGR group,there was no significant difference in BMI,waist circumference,waist-to-hip ratio between the T2 DM group(all P>0.05).Comparison of biochemical measurements:There were no significant differences in liver function indexes such as ALT and AST between the three groups,renal function indexes such as UREA,UA and CREA,and lipid metabolism indexes such as TG,TC,HDL and LDL(both P>0.05).2.Comparison of glucose metabolism related indicators and Asprosin and TNF-α among the three groups.FBG,2h blood glucose,2hINS,glycosylated hemoglobin,HOMA-IR,HOMA-β,Asprosin,TNF-α were significantly different between the three groups(all P<0.001).Compared with the NGR group,FBG,2h blood glucose,2h INS,glycosylated hemoglobin,HOMA-IR,Asprosin,TNF-α in IGR group were significantly increased(both P<0.05),and there was no significant difference in HOMA-β between IGR group(P>0.05);FBG,2h blood glucose,2hINS,glycosylated hemoglobin,HOMA-IR,Asprosin,TNF-α in T2 DM group were significantly increased(both P<0.05),and HOMA-β was significantly decreased in T2 DM group(P<0.05).Compared with the IGR group,FBG,2h blood glucose,glycosylated hemoglobin,HOMA-β,TNF-α in T2 DM group were significantly increased(P<0.05).There was no significant difference in Asprosin and HOMA-IR between T2 DM group(respectively P=0.05,P>0.05).3.Comparison of indicators related to body fat distribution among three groups.There was a statistically significant difference in visceral fat area(VAT)between the three groups of NGR,IGR and T2DM(P=0.012).There was no significant difference in body fat percentage between the three groups(P>0.05).Compared with NGR group,VAT was significantly increased in IGR group and T2 DM group(P<0.05).There was no significant difference in VAT between T2 DM group and IGR group(P>0.05).4.Asprosin and its correlation analysis.Pearson correlation analysis was used to observe the influencingfactors of Asprosin.Asprosin was positively correlated with BMI,FBG,2hblood glucose,HbA1 c,HOMA-IR,VAT and TNF-α(r=0.151,P=0.006;r=0.352,P<0.001;r=0.366,P<0.001;r=0.318,P<0.001;r=0.125,P=0.023;r=0.182,P=0.001;r=0.317,P<0.001,respectively).5.Stepwise linear regression analysis.In the stepwise linear regression.When the covariates associated withAsprosin were re-adjusted,PBG and TNF-α were independently associatedwith Asprosin(P=0.004,P<0.001,respectively).Conclusion:1.Compared with NGR population,serum asprosin levels are significantly elevated in IGR and T2 DM populations,suggesting that it may be a risk factor for glucose metabolism disorders.2.Asprosin is positively correlated with TNF-α and PBG,suggesting that Asprosin may affect glucose metabolism through TNF-α-related pathways,and the secretion of Asprosin may be regulated by blood glucose.