Study Of The Microdevice For Single Cell Capture, Array, Release And On-Chip Fast Staining

Microchip for single cell capture,array and identification has become a powerful tool for single cell study.However,current microfluidics methods for single cell analysis still remain modest and meanwhile fast immunostaining on-chip method has not been explored yet.To optimize the technology,we designed a novel trap structure for single cell capture,array and release with an oscillatory staining method for rapid on-chip cell immunostaining.The baleen-shaped trap structure array is built based on equal distribution of lateral flow in order to achieve single cell array in high velocity and decrease the clogging possibility.In order to make the flow velocity distribute more evenly,we optimize the width of the side channel and the depth of the microchannel.Then we investigate the function of the optimized chip,and the results show the baleen-shaped trap structure can realize single cell capture,array and release in high flow velocity with high efficiency（capture:⁹2.7%and release:⁹9%）.Based on bubble compressibility,we develop a simple oscillator like a RC circuit.A length of glass capillary with a sealed bubble is plugged in the outlet.By applying one periodic air pressure in the inlet,oscillation motion in the chip is generated.In addition,we explore the oscillation mechanism theoretically and experimentally.The oscillation can be easily regulated by changing the length of the capillary.The on-chip mixing time of fluorescein sodium is shortened to 14s through oscillation(P_push0.03MPa,f 1Hz,L 4cm).Combined the novel trap structure and oscillation method,we develop a microchip for single cell study.With an optimized oscillation condition（3MPa,1Hz,3cm）,fast staining is achieved with the advantages of less time（5min）and reagent consumption（2μL）,which is demonstrated through microbeads experiments.Caco-2 cells are used to validate the function of the microdevice.Thus,we develop a simple,flexible and efficient microdevice,which can realize single cell capture,release with high efficiency and fast staining using oscillatory method.We believe it will provide a promising approach for single cell study.