| Salmonella spp.is one of the most common foodborne pathogens and Salmonella enterica serovar Enteritidis(S.Enteritidis)is a predominant serotype in both developing and developed countries.The antimicrobial resistance of Salmonella has been rising year by year.It posed a significant problem to global public health.This study was undertaken to determine antimicrobial susceptibility,virulence gene profiles,genetic relatedness and characterization of plasmids among 119 S.Enteritidis isolates from children in Shanghai aged < 10 years old during 2010-2012.Consequently,the main contents and results in this study are as follows:(1)Among these isolates,71.4%(n = 85)were resistant to sulfisoxazole,59.66%(n = 71)were resistant to ampicillin and 47.05%(n = 56)were resistant to streptomycin.A total of 35 types of antimicrobial resistance patterns,of which 24 were MDR patterns.The positive rates of sul1,sul2 and gyrA genes were 100%.(2)Among all the isolates,70.6%(n = 84)isolates carried all 10 virulence genes.A total of 16 virulence patterns were found.(3)We found 57 PFGE patterns among 92 antimicrobial isolates that were mainly grouped into five clusters(A to E)according to 80% similarity.(4)Among 75 MDR S.Enteritidis isolates,of which 71 contained Inc FIIs and Inc FIC plasmid groups.Antimicrobial-resistant phenotype of 6 isolates were changed after elimination treatment was carried out for 8 isolates by using high-temperature SDS method and their plasmid groups were obviously changed.In summary,this study provides data support for better understanding of the status of Salmonella Enteritidis resistance and the prevalence of plasmids in Shanghai. |
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