The Effct Of Long Intergenic Non-coding RNA 00238 On HBV Replication And Expression In Vitrs

Objective:to investigate the effect of Linc00238 on HBV replication and protein expression;To explore the potential mechanisms.Methods: HBV positive and HBV negative liver tissues were proceeded to LincRNA array,and interested Linc00238 was confirmed further by real-time PCR in HBV expression cell line HepAD38 as well as HBV transient transfection cell line HepG2.Linc00238 and HBV interaction was further determined by gain-of-funtion and loss-of-function study in HepAD38 cells Dual-luciferase reporter assay was used to detect the effect of Linc00238 on promoter activity of HBV.Results:13 LincRNAs expression changed HBV positive liver tissues,including 2 up-regulates and 11 down-regulates.Among of them,Linc00238 expression was significantly down-regulated in HBV infection liver tissue,HBV expression cell line and HBV transient expression cell line(p<0.05).HBV replication and protein levels were significantly inhibted when Linc00238 overexpressed in HepG2(p<0.05),and HBVreplication and protein recoved when sh00238 was introduced.The reporter luciferase reporter system showed Linc00238 had no effect on HBV promoter activity(p>0.05).Conclusions:Through this study we frist comfirmed that Linc00238 could effect on HBV replication and protein expression,which exert its function without affect HBV promoter activities.