| Oudemansiella Furfuracea,also known as O.radicata Var.furfurace,or Colllybia Radicata Var.furfuracea,belonging to Agaricales Tri-cholomataeeae Oudemansiella Speg,also commonly known as Dew chicken gizzard.In the southwest of China,it is widely distributed and artificial cultivation has been initially achieved.Studies have shown that they could benefit to immune system regulation,and anti-oxidation.At present,the research on the genus Oudesteria mainly focuses on the long-rooted mushroom.This article will discuss the physiochemical properties of Oudemansiella furfuracea.The pharmacological effects of polysaccharides.Screening the optimum response surface extraction technology,the basic premise of nutrients to confirm its amino acid content of trace elements and the like,monosaccharide composition analysis and molecular weight distribution.To further study understanding Oudemansiella furfuracea in the role of immune system regulation,anti-inflammation,and protection of the liver,will provide material basis for the development,application,and popularization of the later-day Oudemansiella furfuracea.1.With Water extraction and alcohol precipitation Extract polysaccharides and set up single factor experiments to Response surface method Optimizing the extraction process to obtain Oudemansiella Furfuracea Polysaccharide,OFP.the best way to mention conditions.The results showed that the optimum extraction technology: extraction time of 3 hours,the extraction temperature 80 ℃,extract liquid ratio of 35: 1,and the sugar content of the polysaccharide extraction rate under the optimal conditions was 8.72% and 28.30%,respectively,almost no sugar Aldehyde,protein content 9.40%.2.The Shimadzu HPLC system was used on an Agilent Eclipse XDB-C18 column with a mobile phase of PBS,a flow rate of 1.0 ML/min,an injection volume of 10 μl,and a detection wavelength of 245 nm.After complete acid hydrolysis of the polysaccharides of OFP,compared with the standard,the results showed that the OFP contains a large amount of Glc,and contains a certain amount of Gal and Man,with a small amount of Fuc,GlcA and Xyl.The high molecular weight gel chromatography and LC-10 Avp high performance liquid chromatography system was used to measure the molecular weights of the three fractions of 616 KD,13KD and 2KD.3.The content of amino acid in the polysaccharide of Oudemansiella furfuracea was tested.The results showed that the total amino acid content in the tested polysaccharide was 1.87g/100 g.A total of 18 amino acids were measured,including 8 humans such as THR,TYR and PHE.Amino acids and many other amino acids.In the experiment of measuring the contents of trace elements,the results showed that the polysaccharides contained in the Oudemansiella furfuracea were contained multiple trace elements,including three kinds of essential trace elements such as copper,zinc and iron,and various other trace elements.4.In the immunomodulation experiment of normal mouse with scaly stalk O.obliquus polysaccharide,the acute toxicity test of polysaccharide was conducted with reference to the approximate LD50 method proposed by Muller.The changes of body weight and organ index of mice were observed and the cells in mouse serum were observed.The content of factors was measured.Flow cytometry was used to measure the effect of polysaccharides on lymphocyte subsets of mice spleen.The proliferation of spleen lymphocytes of mice was detected by polysaccharides.The results showed that the LD50 of OFP polysaccharides was > 5g/kg,which complied with national standards.Polysaccharides each dose can improve normal mouse thymus and spleen index,wherein the high dose group for improving performance of thymus extremely significant(P <0.01)the rest of the group of organ index improved but not statistically Meaning.Compared with the blank control group,the high and middle doses of polysaccharides can significantly increase the serum IL-2 level(P<0.01).The serum levels of IL-4 can be significantly increased in high-medium and low-dose groups(P<0.01).The serum levels of TNF-α and IFN-γ were increased(P<0.01).Polysaccharides in the high-and middle-dose groups of spleen lymphocyte subsets significantly increased CD4+ content(P<0.01),and polysaccharide high-dose group significantly reduced CD8+ content(P<0.01).The high and middle doses of polysaccharides can significantly increase the ratio of CD4+/CD8+(P<0.01).Meanwhile,the proliferation of mouse spleen lymphocytes could promote each dose group,and dose-proportional promoting effect,the highest dose group(1000mg / kg)particularly significant(P <0.01).5.Immunosuppression of immunosuppressive mice induced by Cyclophosphamide in the immunosuppressive mice of immunosuppressive mice induced by Cyclophosphamide.The change of mouse organ index,the content of cytokines in serum,the change of mouse peritoneal macrophages Phagocytosis and immunosuppression of mice with red blood cell count and white blood cell count.The results showed that compared with the model group,polysaccharides in each dose group can significantly improve the mouse thymus and spleen index,which in the middle dose group is the most significant(P <0.05),although the other groups have improved,but no statistical significance.Compared with the model group,the high,medium and low doses of polysaccharides can be increased because Immunosuppressed and reduced levels of IL-2,IL-4,TNF-α,and IFN-γ,among which,the recovery of IL-2,IL-4,TNF-α,and IFN-γ in the high dose and middle dose groups of polysaccharides There was a significant difference between the two levels(P<0.01).Compared with the model group,each dose of polysaccharide can improve the phagocytic function of peritoneal macrophages in immunosuppressed mice,especially in the high dose group(P<0.01).Compared with the model group,the high and middle doses of polysaccharides can significantly increase the number of leukocytes in mice(P<0.01),and polysaccharides in the middle dose group can significantly increase the number of red blood cells(P<0.05).6.In alcohol-induced acute liver injury test in mice,each group of blank group,model group,positive group,high,middle and low dosage of polysaccharides was fed with the corresponding solution for 3 hours,except that the blank group was given with saline.The rest of the groups were fed by 56° Beijing Erguotou,continuous modeling 14 days to conduct research.The results showed that the high-and medium-dose group of polysaccharides of OFP can significantly relieve hepatomegaly caused by liver injury,and reduce the thymus index and spleen index.Among them,the effect of high-dose group was especially significant(P<0.01).Polysaccharides can reduce the activity of ALT,AST in serum and increase the ratio of AST/ALT.The effect of high dose group on reduction of ALT is especially significant(P<0.01).Polysaccharides can increase the activity of SOD and GSH-Px in liver tissue and decrease the content of MDA.The activity of SOD and GSH-Px increased significantly in medium and high dose groups(P<0.01).Obvious pathological changes were observed under microscope.improve.7 The anti-inflammatory effect of polysaccharides was studied by using mouse ear edema in the anti-inflammatory test of L.edulis polysaccharides.The results showed that compared with the control group,the high dose of polysaccharides could significantly reduce the degree of ear swelling in mice(P<0.05).The spleen index and thymus index increased significantly,with the high and medium dose groups showing a very significant increase(P<0.01). |
PDF Full Text Request