The Role And Mechanism Of Long Noncoding RNA FBXL19-AS1 In The Development Of Colorectal Cancer

Objective To investigate the expression of long noncoding RNA FBXL19-AS1 in colorectal cancer(CRC)tissues and its underlying clinical value.To investigate the effects of FBXL19-AS1 on CRC cell biological behavior.Further to explore the relationship between FBXL19-AS1 and its justice chain FBXL19 interaction and its effect the mechanism in the Wnt signaling pathway.Methods The expression level of FBXL19-AS1 in 59 colorectal cancer tissues and corresponding tissues adjacent to carcinoma were detected by quantitative reverse transcription(qRT-PCR),the relationship of FBXL19-AS1 with clinical pathological parameters of CRC patients were assessed.To detect the localization of FBXL19-AS1 in CRC cell lines by qRT-PCR and FISH.The relative expression of FBXL19-AS1 in CRC cell lines was detected.Short hairpin RNAi vector targeting FBXL19-AS1 and overexpressed vector were constructed and then transfected into CRC cell lines,the expression of interference detection efficiency was detected.The effect of FBXL19-AS1 on cell proliferation was detected by CCK-8 and colony formation assay.The effect of FBXL19-AS1 on cell cycle distribution and apoptosis was detected by flow cytometry.The effect of FBXL19-AS1 on cell invasion and migration of CRC cells was detected by transwell assay.The expression level of FBXL19 in 59 colorectal cancer tissues and corresponding tissues adjacent to carcinoma were detected by qRT-PCR and analyzed its correlation with FBXL19-AS1.The effect of FBXL19-AS1 on the expression of FBXL19 and the Wnt signaling pathway proteins was detected by western blot.Results The expression of FBXL19-AS1 was significantly higher in cancerous tissues than in adjacent normal tissues,the high expression of FBXL19-AS1 was significantly associated with tumor differention,tumor size,TNM stage and lymph node metsstasis.In the function study of colorectal cancer cell lines,CCK-8 assay showed that the growth rate of CRC cells was significantly slowed down after knockdown of FBXL19-AS1(P<0.05),and the growth was significantly increased when FBXL19-AS1 was overexpressed(P<0.05).Flow cytometry to detect the cell cycle and changes showed G0/G1 phase cell ratio decreased and cell ratio of S phase increased,while FBXL19-AS1 was overexpressed had the opposite effect(P<0.05).Meanwhile,knockdown of FBXL19-AS1 promoted apoptosis in CRC cells(P<0.05).The transwell assay showed that knockdown FBXL19-AS1 could impaired the invasion and migration ability of CRC cells(P<0.05),when the FBXL19-AS1 overexpressed,the ability would enhanced(P<0.05).The expression of FBXL19 was significantly higher in cancerous tissues than in adjacent normal tissues,and was positively correlated with the expression of FBXL19-AS1 by qRT-PCR.Western blot showed that knockwed down FBXL19-AS1 could downregulated FBXL19 expression(P<0.05),and its may also be involved in the regulation of Wnt signaling pathway.Conclusions FBXL19-AS1 was highly up-regulated in CRC tissues and is correlated with tumor differention,tumor size,TNM stage and lymph node metastasis.FBXL19-AS1 can be used as an oncogene,promotes CRC cell migration and invasion by regulating the expression of FBXL19,and FBXL19-AS1 can activate Wnt signaling pathway to promote proliferation,migration and invasion.