The Network Pharmacology Research Of Antithrombotic Mechanism Of Heparin And Ligustrazine

Objective:To study the synergistic mechanism of heparin and ligustrazine in the treatment of thrombotic disease by Network pharmacology.Methods : Searched the target of heparin in PDB Database and made molecular docking;Ligustrazine were used to predict and screen the targets relying on reverse molecular docking server(DRAR-CPI)and the Comparative Toxicogenomics Database.We used Auto Dock Vina to study the the affinity between the ligustrazine and antithrombotic targets.We structured the Protein-protein interactions(PPI)network of the antithrombotic targets of heparin and ligustrazine based on STRING platform.We analyzed KEGG pathways of the antithrombotic targets of heparin and ligustrazine based on biological information annotation databases(DAVID).Results:Heparin induced AT III allosteric?activation and inhibited thrombin.Ligustrazine acted on Coagulation factor X?Coagulation factor IX?Tyrosine-protein kinase JAK2?Coagulation factor VII ?Plasminogen activator inhibitor 1 ? Plasma kallikrein 6 targets.Heparin and ligustrazine realized the function of antithrombus by blocking multiply coagulation cascades pathway in the upstream and downstream.Conclusion : Heparin and ligustrazine acted on multiple Coagulation factor and thrombin ?Plasminogen activator inhibitor and some antithrombotic key target,adjusted coagulation fibrinolytic system,realized the function of anticoagulation and thrombolysis.