Studies On The Chemical Constituents From Garcinia Multiflora Fruits And Their Bioactivities

The research advance of the chemical constituents and biological activities of the fruits of Garcinia multiflora which belong to the Garcinia genus of Guttiferae family has been comprehensively summarized in this dissertation.Total 153 compounds from the stems,branches,leaves and fruits of Garcinia multiflora were sorted and classified,and their pharmacological effects were summarized.Thirty-seven compounds（1-37）were isolated from the fruits of G.multiflora Champ.ex Benth by modern separation technology,including 32 PPAPs（1-32）,and 5other types of compounds（33-37）.Among these compounds,2-3,5-6,8-9,11-21,24-28,30-31 are new compounds.2-3 are type A BPAPs.5-6,8 are type B BPAPs.9,11 are adamantane-type PPAPs.12-21 are homoadamantane-type PPAPs.24-28 are complicated PPAPs via intramolecular[4+2]cycloadditions from MPAPs.30-31 are other types of PPAPs.The anti-inflammatory activity of 29 compounds from the fruits of G.multiflora were determined.The results showed that compounds 1,6,19,22,23 and 26exhibited good anti-inflammatory activity and could inhibit the release of NO.Among them,compound 23（Hyphenrone M）had the best NO inhibitory activity,and its IC₅₀was 12.50±2.31μM.The study of anti-inflammatory activity of PPAPs lays a foundation for finding leading compounds and developing new drugs.The antitumor activities of 29 isolated compounds（1-3,5-14,16-20,22-32）were evaluated against gastric cancer cell SGC7901,hepatoma carcinoma cell HepG2,colon cancer cell HCT-116 by CCK-8 method.The results showed that part of PPAPs showed good anti-tumor activity on human gastric cancer cell SGC7901.Compounds 1,20,23 showed the same anti-tumor activity as positive control drug cisplatin.The best active compound is 1（Hyperscabrone M）,and its IC₅₀ value is 7.66μM.The IC₅₀ values of the tested compounds in human hepatoma carcinoma cell HepG2 were similar to those of the positive drugs,including 20,23 compounds.Compound 20（Garcimultinone P）have better activity and its IC50 value is 17.84μM.In the experiments targeting human colon cancer cell HCT-116,only the compound23（Hyphenrone M）was found to be equivalent to that of the positive drug with IC₅₀value of 18.07μM.It suggested that PPAPs could be used as an antitumor drug lead compound for further research and development.