Expression Of HENT1 And G9a In Childhood Acute Lymphoblastic Leukemia

Objective: This study aims to detect the expression level of human equilibrative nucleoside transporter 1(h ENT1)and histone methyltransferase G9a(G9a)in childhood acute lymphoblastic leukemia(ALL),explore the role in the pathogenesis of primary childhood ALL and analyze the relationship between expression level and risk classification,fusion gene and induction remission.Methods: Seventy-nine non-superdiploid primary children with bone marrow(BM)specimens were used as experimental group.Sixteen patients with idiopathic thrombocytopenic purpura(ITP)bone marrow samples were used as control group.The expression levels of h ENT1 and G9 a genes in bone marrow samples of ALL and ITP patients were detected by real-time fluorescent quantitative PCR.Results:1.The expression level of h ENT1 in ALL group was significantly higher than that in ITP group.The difference was statistically significant(P <0.05).2.There was a statistically significant difference in the expression level of h ENT1 between low-risk and high-risk groups(P<0.05).3.The expression level of G9 a in ALL was significantly higher than that in ITP group,and the difference was statistically significant(P<0.05).4.There was no correlation between h ENT1 and G9 a expression levels(r=-0.064,P=0.572).5.There was no significant difference between h ENT1 and G9 a expression levels and ALL-induced remission rate and fusion gene(P>0.05).Conclusion:1)The expression levels of h ENT1 and G9 a in children with primary ALL are significantly increased.2)The expression level of h ENT1 is related to the risk classification,and the higher the risk classification is,the higher the expression level is.3)Our study suggests that both h ENT1 and G9 a may be associated with the pathogenesis of ALL,and h ENT1 is expected to be an indicator for evaluating the risk classification of ALL.