Pathological Characters And Molecular Pathogenesis Of Diabetic Neuropathic Osteoarthropathy

Objective: The aim of current study was to observe the pathological characters and relative molecular pathogenesis of diabetic neuropathic osteoarthropathy(DNOAP).Methods: The pathological and ultrastructural changes were observed with use of staining and electron microscope.The cultured chondrocytes were separated to DNOPA group and normal control group,and the RANKL,OPG,IL-1β,IL-6,TNF-α,Aggrecan,intracellular ROS level and the incidence of apoptosis.Results: Compared with the normal cartilage,the hyaline cartilage was strip-like arranged,chondrocytes were reduced,subchondral bone is proliferated,and the structure is disordered;and massive osteoclast formation in subchondral bone region.The mitochondrial in DNOAP chondrocytes were swelling,membrane structure incomplete,and disordered arrangement.And,the endoplasmic reticulum was swollen,and the nucleus became enlarged,and the chromatin was partially broken and concentrated at the edge of the nuclear membrane.The expression of RANKL,IL-1β,IL-6 and TNF-α were higher in DNOAP group(P<0.05).The OPG and Aggrecan expression were lower in DNOAP group(P<0.05).The intracellular ROS level was significantly higher in DNOAP group(P<0.05).And the incidence of apoptosis in DNOAP group was significantly higher than normal control(P<0.05).Conclusions: The cartilage pathological characters were much different compare with normal,and with serious cytoplasmic organoids damage.RANKL and related inflammatory factors were high expression in the DNOAP chondrocytes.These results suggested that DNOAP is character with cartilage structure change and cell damage,and inflammatory reaction plays a role in pathogenesis.