LncRNA IGFBP7-OT Promotes The Occurrence And Development Of Osteoarthritis Via Up-regulating IGFBP7

Objective: The present study was aimed to investigate the functions and underlying mechanisms of lncRNA IGFBP7-OT in the occurrence and development of osteoarthritis(OA).Methods: Human articular cartilage were identified by immunohistochemical and Saffron O staining.Real-time PCR and Western blot analysis were performed to detect the expressions of lncRNA IGFBP7-OT and IGFBP7 in cartilage tissues or primary chondrocytes.The cell proliferation was evaluated by CCK8 assay.The apoptosis was measured by flow cytometry analysis.The apoptosis related-factors(PARP/Caspase-3/Caspase-9/Bax/Bcl2/Suivivin)were detected by Western blot.Results: LncRNA IGFBP7-OT and IGFBP7 were highly expressed in OA cartilage.Overexpression of IGFBP7-OT and IGFBP7 accelerated the degradation of extracellular matrix(ECM),respectively.Meanwhile,overexpression of IGFBP7-OT and IGFBP7 inhibited the proliferation and promoted the apoptosis of primary chondrocytes.Moreover,IGFBP7-OT positively regulated the mRNA and protein expression of IGFBP7.And the effects of IGFBP7-OT on ECM degradation as well as cell proliferation and apoptosis of primary chondrocytes were attenuated by knockdown of IGFBP7.Conclusions: LncRNA IGFBP7-OT and IGFBP7 were upregulated in the articular of OA cartilage.Overexpression of IGFBP7-OT aggravated the degradation of ECM,inhibited the proliferation and induced the apoptosis of chondrocytes by upregulation of IGFBP7.These results suggest that IGFBP7-OT and IGFBP7 play important roles in the occurrence and development of OA,and they may serve as novel targets for the prevention and treatment of OA.