The Expression And Significance Of Autophagy Associated Proteins In Peripheral Blood Monocytes In Patients With Acute Myocardial Infarction

ObjectiveTo detect the expression levels of autophagy-associated proteins LC3-I,LC3-II and Beclin 1 in peripheral blood mononuclear cells in patients with acute myocardial infarction,and to analyze its significance.MethodsWe recruited 62 cases of patients with acute myocardial infarction(AMI group)and 56 individuals with stable angina pectoris(SAP,control group)for the study.Collection of monocytes from their peripheral blood.The expression levels of MAP1-LC3 were detected by immunofluorescence.The expression levels of LC3-I,LC3-II and Beclin 1 were detected by Western blotting.Then compare with the difference of onset time of acute myocardial infarction,Gensini scores,and the autophagy level and autophagic flux before and after PCI.To analyze the significance of peripheral blood monocyte autophagy in acute myocardial infarction.Results1.Compared with the control group,the number of green fluorescent spots in peripheral blood mononuclear cells was increased significantly in acute myocardial infarction,and the levels of LC3-I,LC3-II and Beclin 1 were significantly increased(P< 0.05).2.The longer the onset time of acute myocardial infarction,the higher the levels of LC3-I,LC3-II and Beclin 1,and the autophagic flux was significantly blocked after the onset time>24h(P<0.05).3.Compared with the low Gensini score(<40 points)group,the levels of LC3-I,LC3-II and Beclin 1 were significantly increased and the autophagic flux was blocked in the high Gensini score(≥ 40 points)group(P<0.05).4.Compared with preoperative PCI,the levels of LC3-I,LC3-II and Beclin 1 were significantly decreased after PCI in acute myocardial infarction,and the impaired autophagy flux was also restored(P <0.05).ConclusionThe level of autophagy in peripheral blood mononuclear cells is significantly increased in acute myocardial infarction.The longer the onset time of acute myocardial infarction or the coronary lesions are severer,the higher the level of the autophagy,andthe autophagy flux is also significantly blocked.After PCI treatment,the level of autophagy decreased and the autophagic flux is blocked.Autophagy may be involved in the development and progression of acute myocardial infarction.Proper regulation of autophagy in peripheral blood mononuclear cells and improvement of autophagy flux may be therapeutic targets for improving myocardial cell injury after acute myocardial infarction,reducing acute coronary events and the risk of death.