Objective: To explore whether sodium hydrosulfide can resist the decrease of mitochondrial membrane potential(MMP)and the activation of mitochondrial membrane permeability transition pore(MPTP)in epidermal cells by ATP sensitive potassium channel.Methods: In vitro culture of neonatal 1 day SD suckling mouse dorsal epidermal cells to the 3rd generation.The abdominal aortic blood of normal male SD rats(350-400g)and burned male SD rats were centrifuged and labeled as normal rat serum and burned rat serum respectively.The serum was filtered with 300 mesh filter.The serum of normal rat and burned rat was used to prepare 10% normal serum complete culture medium and 10% burned rat serum complete culture medium for reserve.The intervention concentration of sodium hydrosulfide was 150 micromol/L and glibenclamide was 200 micromol/L.The experiment was divided into five groups: normal serum group,burn serum group,burn serum + sodium hydrosulfide(NaHS)group,burn serum + glibenclamide(Gli)group,burn serum + glibenclamide + sodium hydrosulfide group.Each group interfered with the third generation of suckling mouse epidermal cells cultured in vitro.After intervention for 4 hours in each group,the cells were treated according to the mitochondrial membrane potential and the instructions of mitochondrial membrane permeability transition pore kit.Changes of mitochondrial membrane potential and mitochondrial membrane osmotic transition pore in epidermal cells of each group were detected by flow cytometry.Results: At the same time point in different periods,the following conclusions can be drawn by measuring the percentage of mitochondrial membrane potential reduction(Q4)and the percentage of membrane permeability transition pore inhibition overactivation(P2)of epidermal cells after intervention in each group.(1)Q4 value in normal serum group < burn serum group,P2 value in normal serum group > burn serum group.(2)Q4 value of burn serum + NaHS group < burn serum group,P2 value of burn serum + NaHS group > burn serum group.(3)Q4 value of burn serum group < burn serum + Gli group,P2 value of burn serum group > burn serum + Gli group.(4)Q4 value of burn serum + NaHS group < burn serum + Gli + NaHS group,P2 value of burn serum + NaHS group > burn serum + Gli + NaHS group.The above results were P<0.05,and the difference data analysis had statistical significance.Conclusions:(1)Microamounts of sodium hydrosulfide mediate KATP channel to reduce the mitochondrial membrane potential decrease after epidermal cell injury and reverse the damage of epidermal cells after burn.(2)Microamounts of sodium hydrosulfide mediate KATP channel to reduce the activation of mitochondrial membrane permeability transition pore and reverse the injury of epidermal cells after burn injury. |