The Role And Mechanism Of Schistosoma Japonicum Peptide SJMHE1 In Asthmatic Mice

PART I Effect of schistosoma japonicum peptide SJMHE1 in asthmatic mice[Objective]To investigate the effect of schistosoma japonicum peptide SJMHE1 on airway inflammation in asthmatic mice[Method]Male Balb/c mice(6 to 8 weeks old)were randomly divided into four groups:PBS group,ovalbumin(OVA)group,OVA/PBS group and OVA/SJMHE1 group.Asthmatic mice were established using sensitization and challenging in OVA,OVA/PBS and OVA/SJMHE1 group.Hematoxylin and eosin(HE)staining was used for testing the lung pathological changes in mice.Serum anti-OVA-specific immunoglobulin E(IgE)level was detected by ELISA Hemocytometer was used to count the number of BALF cells[Result]Compared to the PBS group,OVA and OVA/PBS groups showed the increase of the inflammatory cell infiltration,the number of BALF cells and eosinophils,and the IgE product.Compared to OVA or OVA/PBS group,OVA/SJMHE1 group induced the decrease of the inflammatory cell infiltration,the number of BALF cells and eosinophils,whereas OVA/SJMHE1 group provided almost no reduction in serum IgE level in the spleen[Conclusion]Taken together,our results indicated that Schistosoma japonicum peptide SJMHE1 can reduce airway inflammation in asthmatic micePART II Schistosoma japonicum peptide SJMHE1 corrects the balance of Thl/Th2/Th17/Treg in asthmatic mice[Objective]The present study is designed to determine whether schistosoma japonicum peptide SJMHE1 has immunoregulatory effects on the(helper T(Th))Thl/Th2/Thl7/regulatory T cell(Treg)balance in asthmatic mice.[Method]Male Balb/c mice(6 to 8 weeks old)were randomly divided into four groups:PBS group,ovalbumin(OVA)group,OVA/PBS group and OVA/SJMHE1 group.Asthmatic mice were established using sensitization and challenging in OVA,OVA/PBS and OVA/SJMHE1 group.The production of pro-inflammatory and anti-inflammatory cytokins in splenocytes and lungs were measured by real time quantitative PCR(qPCR).The proportion of Thl/Th2/Thl7/Treg in the spleenocytes was detected by flow cytometry.Furthermore,The expression of GATA3 and RORyt in the lung tissue were measured by western blotting and the expression of Foxp3 in the lung tissue was detected by immunohistochemistry[Result]We showed that SJMHE1 treatment modulated the production of pro-inflammatory and anti-inflammatory cytokins in splenocytes and lungs of allergic mice,reduced the percent of Th2 cells,and increased the proportion of Thl and Treg cells,accompanying by the increase of Foxp3 expression,and the decrease of GATA3 and RORyt in lungs of allergic mice[Conclusion]The findings provide evidence that SJMHE1 can intervene the development of asthma for diminishing its airway inflammation in mice.The immunological mechanism may involve the down-regulation of Th2 and Th17 response and up-regulation of Thl and Treg responsePART III Schistosoma japonicum peptide SJMHE1 reduces the number of myeloid-derived suppressor cell in asthmatic mice[Objective]To investigate the effect of schistosoma japonicum peptide SJMHE1 on MDSC in asthmatic mice[Method]Male Balb/c mice(6 to 8 weeks old)were randomly divided into four groups:PBS group,ovalbumin(OVA)group,OVA/PBS group and OVA/SJMHE1 group.Asthmatic mice were established using sensitization and challenging in OVA,OVA/PBS and OVA/SJMHE1 group.The proportion of PMN-MDSCs and M-MDSC in the spleenocytes was detected by flow cytometry and the expression of MDSC in the lung tissue was detected by immunohistochemistry[Result]Compared to the PBS group,OVA and OVA/PBS groups showed the increase of the percent of PMN-MDSC in the spleen,and the number of MDSC in the lung of mice,whereas M-MDSC in the spleen showed no significant changes.Compared to OVA or OVA/PBS group,OVA/SJMHE1 group induced the decrease of the proportion of PMN-MDSC in the spleen,and the number of MDSC in the lung of mice,whereas OVA/SJMHE1 group provided almost no reduction in M-MDSC in the spleen.[Conclusion]Schistosoma japonicum peptide SJMHE1 may suppress airway inflammation in asthmatic mice by reducing the number of MDSC.