Effect Of Biological Characteristics Of Oral Squamous Cell Carcinoma Cells By Inhibiting The Expression Of Focal Adhesion Kinase

Objective: To study the effect of inhibiting the expression of focal adhesion kinase(FAK)on oral squamous cell carcinoma(OSCC)cells,and to explore the killing effect of x-ray irradiation on OSCC cells when the expression of FAK is inhibited,so as to provide a theoretical basis for the selection of clinical treatment options for OSCC in the future.Materials and Methods: Focal adhesion kinase inhibitor were used to inhibit oral squamous cell carcinoma cell line CAL27.X-ray irradiation was provided by the irradiation terminal of the Institute of Modern Physics of Lanzhou Branch of the Chinese Academy of Sciences..Western Blot was used to detect the expression of related proteins,real-time unlabeled cell analysis system,clone formation test and Ki-67 immunofluorescence staining were used to detect the proliferation activity of cells in each experimental group,Transwell migration test and wound healing test were used to detect the migration ability of cells,and Annexin V/PI double staining was used to detect the apoptosis of cells in each experimental group.The changes of cytoskeleton and mechanical properties of cells were examined by fluorescence staining and atomic force microscopy.Results: When CAL27 cells were treated with the focal adhesion inhibitor,the proliferative activity was decreased and the migration ability was significantly inhibited compared with the normal control cells.Compared with the normal control group,the Young’s modulus increased,and the cytoskeleton became less and irregular.After the addition of X-ray irradiation,the inhibitor group showed a higher apoptotic rate.The ability of proliferation and migration of inhibitor plus x-ray irradiation was the lowest in all groups.Conclusion: Focal adhesion kinase inhibitor treatment can effectively reduce the proliferation and migration ability of CAL27 oral squamous cell carcinoma cells,whether or not x-ray irradiation is added.Under x-ray irradiation,CAL27 cells interfered by focal adhesion kinase inhibitor are more prone to apoptosis than those in untreated control group.