Adeno-Associated Virus Mediated Neuropeptide Y Overexpression In Neuropathic Pain

OBJECTIVE: To explore the key genes in animal of neuropathic pain in time series;the effect of adeno-associated virus tool overexpressing neuropeptide Y of neuropathic pain,and its effect on the treatment of pain.METHODS: 1.Analyze the neuropathic pain-related rat gene chip in GEO open dataset,key gene expression was analyzed for related functions,tested in other data sets.2.Adult Sprague-Dawley rats were randomly divided into 4 groups.Three groups were intraspinally injected with neuropeptide Y overexpressing gene adeno-associated virus,negative control virus or normal saline,then selectively ligating the sciatic nerve;sham operation group was only sutured after incision.All animals were tested for BBB score and mechanical pain threshold at specific time points.After the measurement,the L5 spinal cord was taken and the expression of neuropeptide Y was determined by immunohistochemistry.RESULTS: A total of 738 key differentially expressed genes was recognized,including Neuropeptide Y.The expression of neuropeptide Y in the adeno-associated virus group was detected.Neuropeptide Y immunization could be detected after 14 days.NPY overexpressed rats’ mechanical pain thresholds were also increased(P<0.05).CONCLUSIONS: 1.More than 700 genes,such as neuropeptide Y,are the key regulators of neuropathic pain;2.Neuropeptide Y mediated by adeno-associated virus is stable expressed in rat spinal cord tissue;3.Adeno-associated virus-mediated overexpression of neuropeptide Y in rat spinal cord tissue increases the mechanical pain threshold.