The Research On Measurement And Transformation Of Active Components In Epimedium Wushanese T.S. Ying

Epimedium is a traditional Chinese medicine.It has the traditional effect of “tonifying kidney,strengthening bones and muscles”.The Chinese Pharmacopoeia records a total of five Epimediums,including Epimedium brevicornum Maxim,Epimedium sagittatum Maxim,Epimedium pubescens Maxim,Epimedium koreanum Nakai and Epimedium wushanense T.S.Ying.Among them,Epimedium brevicornum Maxim,Epimedium sagittatum Maxim,Epimedium pubescens Maxim and Epimedium koreanum Nakai,are mainly classified into a class of plants with icariin content greater than 0.50%.Epimedium wushanense T.S.Ying is classified into another category because its main component is epimedin C and the content of icariin less than 0.50%.Icariin is the main active ingredient of Epimedium,and has a variety of pharmacological effects,including kidney,impotence,rheumatism,anti-diabetes,anti-cardiovascular disease,anti-cancer and so on.The price of Epimedium on the market is related to the content of icariin.Epimedium wushanense T.S.Ying has low price and low market utilization due to low content of icariin.Therefore,how to improve the content of icariin in Epimedium wushanense T.S.Ying is worth studying.The main contents of this paper are as follows:(1)Effects of planting harvesting and storage on the main active constituents of Epimedium wushanense T.S.Ying.After the establishment of Epimedium wushanense T.S.Ying analysis method,the effects of different medicinal parts,different harvesting months and different storage methods on the content of epimedin C and icariin were studied.After measurement and calculation,when the active ingredient icariin content is the most,the suitable application site of Epimedium wushanense T.S.Ying is the leaf,the best harvesting month is September,and the best storage method is dry.(2)Effect of processing on the main active ingredients of Epimedium wushanense T.S.Ying.The glycosides in Epimedium are affected by the heating temperature and heating time.In this paper,the content of main active components in Epimedium wushanense T.S.Ying extracts and leaves was studied at 140,160,and 180°C,heating time 10,30,50,70,and 90 min.At the same temperature,the content of epimedin A,epimedin B,epimedin C decreased with the increase of heating time,and the content of icariin and baohuoside I increased first and then decrease with the increase of heating time.At 140°C,50,and 70 min,160°C,10,30,and 50 min,180°C,10 min,the content of icariin in Epimedium wushanense T.S.Ying was more than 0.50%.The content of icariin higher than the standard of China Pharmacopoeia.In this paper,the leaves of Epimedium wushanense T.S.Ying containing high content of icariin were obtained by heat treatment.(3)Effect of enzyme transformation on active components of Epimedium wushanense T.S.Ying.Icariin is a derhamnosylation substance of epimedin C.The difference of them is epimedin C contains two rhamnose groups at the position of C-3,but icariin has one rhamnose.In this paper,α-L-rhamnosidase was used to transform epimedin C and the extracts of Epimedium wushanense T.S.Ying.At the optimum reaction temperature and optimum pH,the conversion rate of epimedin C to icariin was 95%.The content of icariin in the Epimedium wushanense T.S.Ying extract changed from 3.50% to 14.63%.In this paper,the extracts of Epimedium wushanense T.S.Ying containing high content of icariin were obtained by enzyme hydrolysis.In this paper,the components of Epimedium wushanense T.S.Ying were studied from the aspects of planting,storage,processing and composition transformation.The best planting and storage conditions,the best processing method and the best enzyme conversion method were determined.In this paper,the content of icariin in Epimedium wushanense T.S.Ying was improved,which provided a method for further medicinal research of Epimedium wushanense T.S.Ying.