Establishment Of Multiple Detection Methods For Tumor Markers

As a kind of disease that endangers human health,tumors have high concurrency rate and high mortality rate,and improve the early diagnosis level of cancer patients,which can effectively prolong life expectancy and reduce mortality.A specific substance produced in tumor cells that can be used for early diagnosis and prognosis of tumors,and is called a tumor marker.The detection method of tumor markers has been a hot topic in recent years.In this paper,neuron-specific enolase was used as an experimental sample to explore its detection method.Neuron-specific enolase(NSE)is a relatively common tumor marker that is clinically used to detect small cell lung cancer and can also be used in the detection of neuroblastoma.There are not many researches on the detection of NSE in China.Therefore,this study has established three detection methods,which provide experiments for clinical diagnosis,evaluation and treatment of small cell lung cancer and neuroblastoma,and preparation of kits and room basis.This subject established three detection methods of NSE,namely enzyme-linked immunosorbent assay,immunoturbidimetric assay and magnetic microparticle immunochemiluminescence method,to explore the best experimental conditions of these three methods to enhance the detection effect and achieve The quantitative measurement of the tumor marker NSE was carried out,and the linear range,sensitivity,specificity and inter-assay intra-assay coefficient of variation of the three established detection methods were evaluated.The final test results show that the linear correlation equation of the ELISA double-anti-sandwich method established in this paper is y=1.0335x-0.0133,the correlation coefficient R~2=0.9976,and the linearity is good in the concentration range of6.25-200ng/mL.The positive sample is at 450nm.The critical value is 0.4786,which does not cross-react with other interfering substances,and has high sensitivity.The NSE antigen can be detected at a concentration of 5ng/mL.The coefficient of variation of the same batch and different batches is less than 5%,and the recovery rate is 98.3%.Has clinical value.This study established an immunoturbidimetric method and plotted a linear correlation curve.It has a good linear curve when the NSE antigen concentration is 6.25-200ng/mL.The linear equation is y=0.0036x+0.7291,R~2=0.992,and the specificity is good.The sensitivity is high,and the NSE antigen of 1 ng/mL concentration can be detected at 560nm.The recovery rate of the method is about 98.9%,and the coefficient of variation between the same batch and different batches is less than 5%by calculation.Based on the double-anti-sandwich method,the NSE magnetic particle chemiluminescence immunoassay was established.The linear equation y=373.81x+13207,the linear correlation coefficient R~2=0.9959,the linear concentration of NSE antigen was 6.25-200ng/mL,and the specificity was strong.The repeatability is good and the sensitivity is high.The detection limit of NSE is 0.5 ng/mL,the average recovery rate is 99%,and there is no"HOOK"effect.