Effects Of Deoxynivalenol And Combined With T-2 Toxion On Liver CYP2E1 In Rats

ObjectiveTo observe the effect of Deoxynivalenol and Combined with T-2 Toxin on Liver Microsomal CYP2E1 in Rats and the damage of organization organs such as liver,kidney,esophagus and intestinal,to explore metabolic pathway and characteristics of mycotoxins.MethodWe randomly assigned 30 young(4weeks)Wistar healthy male rats each into control: DON,DON+T-2,and control group.The mice of DON dosage treat-ment groups were taken orally administered with 0.33μg/g,the mice of DON and T-2 dosage treatment groups were taken orally administered with 0.22μg/g and 0.1μg/g,the same amount of conventional solid particle feed of the rats in control groups.Preparation of liver microsome were through ELISA tests to detect the rat liver microsomal CYP2E1 enzyme content and activity,after 15 days and 30 days.Against rats,respectively in liver,kidney,intestine,esophageal tissues and organs,such as making paraffin section,HE staining method is adopted to improve the dyeing.SPSS 20.0 was used for statistical analysis.The quantitative data was used((?)±s)to describe,statistical methods using linear regression,the quantitative data between DON and control was analyzed by independent samples t test;in the control group,DON group and “DON+T-2” group,data were analyzed with one-way ANOVA,compares the one and one use LSD methods,the results are P≤0.05.Result1.Clinical symptoms: physical development of control rats,not vomiting symptoms.intake down,weight loss,the activity decreases,coat is not whole,xi lie lie prone,ataxia,unresponsive,feces rare stiff and other symptoms in the DON toxin and “DON+T-2”group.2.The results of CYP2E1 enzyme content: The content of CYP2E1 was 6.052±0.124(ng/ml)and 3.595±0.152(ng/ml)in the control group and DON group after 15 days’ contamination.The content of CYP2E1 was 4.511 ± 0.137(ng/ml),2.936±0.093(ng/ml) and 2.484±0.149(ng/ml)in the control group,DON group and “DON+T-2”group after 15 days’ contamination.3.The results of CYP2E1 enzyme activity: The activity of CYP2E1 was 3.541±0.070(U/ml),3.318±0.047(U/ml)and 3.229±0.016(U/ml)in the control group,DON group and “DON+T-2” group after 30 days’ contamination.4.Pathological section observation: Pathological changes in autopsy Autopsy found mice kidney enlargement in DON toxin group,white size focal necrosis of liver surface,control group mice organs found no apparent change.Histopathologic changes Regular control group of liver cells in mice with liver blood sinus radial alignment,centered in central vein into the surrounding liver cell structure in good condition,large,round nucleus and normal tissue,obvious nucleolus,abundant cytoplasm,many basophilic.Pathological biopsy showed that the liver cell of DON group was cytoplasm shallow dye and pink material,the liver cell of “DON+T-2 ”toxin group was edema focal necrosis,and visible liver nucleus pycnosis,hyperchromatic with inflammatory cells infiltration around blood vessels,visible blood vessels set of phenomena,cytoplasm irregular cavity.Kidney on control group,the vision of glomerular structure is normal,renal tubular epithelial cell nuclei and rounded,located at the base,neatly arranged,no obvious pathological changes;DON group view visible protein tube type and part of the malpighian tube cavity with pink mucus effusion protein;“DON+T-2”view visible in protein toxin group kidney type tube,part of the renal small tube cavity with pink protein mucus,visible inside of bowman’s capsule drip pink protein.There is no obvious pathological changes of esophageal tissue on control group;esophageal epithelial thickness of cuticle hyperkeratosis,visible squamous cell hyperplasia is apparent,feed tube wall thickening on DON group;“DON+T-2”toxin groups view esophageal epithelial thickness of cuticle,hyperkeratosis,clearly visible squamous epithelial hyperplasia.Conclusion1.after 15 days,compared with the control group,the content of CYP2E1 in DON toxin group decreased.The result indicated,DON toxin have toxic effect on liver cells,make liver damage,liver metabolism function abate,liver metabolism enzyme is damaged,show the CYP2E1 enzyme content reduced.2.after 30 days,compared with the control group,the content and activity of CYP2E1 in DON toxin group decreased;the content and activity of CYP2E1 in “DON+T-2” toxin group decreased obviously.The result indicated,DON toxin have toxic effect on liver cells,make liver damage,liver metabolism function abate,liver metabolism enzyme is damaged,show the CYP2E1 enzyme content and activity reduced.Compared with the DON toxin group,“DON+T-2” toxin group decreased enzyme content effect more apparent.May be DON toxin and T-2 toxin generate synergy or additive effect,increases the damage of the liver.3.Pathological biopsy showed that compared with the liver of control group,the liver cell of DON group was cytoplasm shallow dye and pink material,the liver cell of “DON+T-2 ”toxin group was edema focal necrosis,and visible liver nucleus pycnosis,hyperchromatic with inflammatory cells infiltration around blood vessels,visible blood vessels set of phenomena,cytoplasm irregular cavity.Kidney,compared with the control group,DON group view visible protein tube type and part of the malpighian tube cavity with pink mucus effusion protein;“DON+T-2”view visible in protein toxin group kidney type tube,part of the renal small tube cavity with pink protein mucus,visible inside of bowman’s capsule drip pink protein.Compared with the control group,DON group view visible esophageal epithelial hyperkeratosis,corneous layer thickening,and squamous epithelial hyperplasia.“DON+T-2”toxin group of esophageal epithelium is hyperkeratosis,corneous layer thickening,feed tube wall thickening,has obvious squamous epithelial hyperplasia.Compared with the control group,DON group in intestinal submucosa edema,vision clearance increases,partial edema of intestinal epithelial cells.“DON+T-2”toxin group visible intestinal mucosa lamina propria tissue edema,intercellular space increases,part of the intestinal mucosa epithelial cells necrosis falls off.