Differences Of FSH,LH,E₂ And β-Ep,CGRP In The Syndrome Of Kidney-yang Deficiency And Kidney-yin Deficiency In Menopausal Syndrome

Objective:（1）The specificity of serum FSH,LH,E₂,β-Ep and CGRP in postmenopausal women and postmenopausal syndrome patients was investigated through the detection and comparison of the relative indexes between postmenopausal women and normal perimenopausal women and normal women of childbearing age.The specificity and correlation of serum FSH,LH,E₂,β-Ep and CGRP in patients with different degrees of disease and symptoms of hectic fever and sweating were also investigated.（2）The differences of serum FSH,LH,E₂,β-Ep and CGRP among patients with kidney Yang deficiency syndrome and kidney Yin deficiency syndrome of menopause syndrome and kidney Yin deficiency syndrome were investigated through the detection and comparison of the relative indexes of the patients with kidney Yang deficiency syndrome and kidney Yin deficiency syndrome of menopause syndrome,so as to provide some objective basis for the microscopic syndrome differentiation of menopause syndrome in traditional Chinese medicine.Methods:The first experiment:According to the disease diagnostic criteria,80 patients with menopause syndrome were included as the study group,and 30 normal women of childbearing age and 30normal women of perimenopausal period were included in the control study.Their serum FSH,LH,E₂,β-Ep and CGRP were detected,and SPSS24.0 software was used for statistical analysis to compare their differences.According to the disease degree evaluation criteria（Kupperman score scale）and the degree of hectic fever and sweating（hectic fever and sweating score scale）,80 patients with menopause syndrome were divided into mild,moderate and severe degrees,and the serum indexes of patients with different degrees were compared.The second experiment:With reference to the disease and syndrome diagnosis criteria,30 cases of patients with kidney Yang deficiency syndrome and kidney Yin deficiency syndrome of menopause syndrome were included.Their serum FSH,LH,E₂,β-Ep and CGRP were detected.SPSS24.0 software was used for statistical analysis to compare the differences between the two syndromes of kidney Yin deficiency syndrome and kidney Yang deficiency syndrome.Results:The first experiment:（1）The general information:According to the Kupperman rating scale,the degree of disease was divided into mild,moderate and severe.It was found that 80 patients in the menopausal syndrome group were distributed in mild and moderate levels,among which 52 were mild,accounting for 65%,and 28 were moderate,accounting for 35%.According to the scale of hectic fever and sweating,the symptoms of hectic fever and sweating were classified as mild,moderate or severe.The results showed that the symptoms of hectic fever and sweating in the 80 patients with menopause syndrome group were mainly moderate,among which 14 were mild,accounting for 17%.Moderate 56cases（70%）;There were 10 severe cases,accounting for 13%.（2）Serum levels of FSH,LH and E₂ were found in patients with menopausal syndrome,normal perimenopausal women,and women of childbearing age:（1）Serum FSH（44.697±44.785）and LH（20.009±18.264）in the normal perimenopausal group were significantly higher than those in the normal perimenopausal group（5.321±1.778）and LH（3.997±2.070）,while E₂（56.279±53.982）were significantly lower than those in the normal perimenopausal group（64.453±36.440）,with statistically significant differences（p<0.05）.The serum levels of FSH（72.296±36.223）and LH（31.177±16.765）in the menopausal syndrome group were significantly higher than those in the normal perimenopausal group,and E₂ level（33.559±15.624 in the menopausal syndrome group;Normal perimenopausal group:56.279±53.982）obviously decreased,and the difference was statistically significant（p<0.05）.（2）Serum FSH（mild:69.464±31.377,moderate:77.557±43.992）and E₂（mild:32.889±13.554,moderate:34.804±19.098）of the patients with menopause syndrome with different disease degrees showed an increasing trend with the aggravation of the disease degree,but the difference was not statistically significant（p>0.05）.LH（mild:31.239±16.625,moderate:31.063±17.328）showed no significant change.The correlation analysis showed that there was no significant linear correlation between the disease degree score of menopause syndrome and the serum levels of FSH,LH and E₂（p>0.05）.（3）Serum FSH（mild:69.557±34.045,moderate:71.541±36.753,severe:80.360±38.741）,E₂（mild:31.821±13.703,moderate:33.161±16.048,severe:36.060±17.269）of the menopausal syndrome patients with different degrees of hectic fever and sweating increased with the aggravation of hectic fever and sweating,but the difference was not statistically significant（p>0.05）.LH（mild:31.098±18.573,moderate:31.696±17.219,severe:28.380±12.106）showed no significant change.The correlation analysis showed that there was no significant linear correlation between hectic fever and sweating and FSH,LH and E₂ levels（p>0.05）.（2）Serum levels ofβ-Ep and CGRP in patients with menopausal syndrome,normal perimenopausal women and women of childbearing age:（1）The serumβ-Ep of the normal group during the perimenopausal period（0.415±0.023）was significantly lower than that（0.494±0.053）of the normal group during the childbearing age,and the difference was statistically significant（p<0.05）.Serum CGRP（0.420±0.023）in the normal perimenopausal group was significantly higher than that in the normal perimenopausal group（0.319±0.031）,and the difference was statistically significant（p<0.01）.The serumβ-Ep（0.333±0.033）of the patients in the menopausal syndrome group was significantly lower than that in the normal perimenopausal group,and the difference was statistically significant（p<0.01）.CGRP in the menopausal syndrome group was significantly higher than that in the perimenopausal normal group（0.498±0.048）,and the difference was statistically significant（p<0.01）.（2）The serumβ-Ep of patients with menopause syndrome of different disease degrees（mild:0.334±0.030,moderate:0.329±0.037）and CGRP（mild:0.506±0.048,moderate:0.484±0.044）showed a decreasing trend,and the difference was not statistically significant（p>0.05）.Moreover,there was no significant linear correlation between the disease degree score of menopause syndrome and patients’serumβ-Ep and CGRP（p>0.05）.（3）The serumβ-Ep of patients with menopause syndrome with varying degrees of hot flashes and sweats showed an upward trend（mild:0.315±0.037,moderate:0.336±0.032,severe:0.339±0.018）,and the difference was not statistically significant（p>0.05）.CGRP（mild:0.507±0.040,moderate:0.499±0.049,severe:0.481±0.047）showed a decreasing trend,and the difference was not statistically significant（p>0.05）.However,there was no significant linear correlation between the score of hectic fever and sweating and the level ofβ-Ep（p>0.05）.However,there was a weak negative correlation between the score of hectic fever and sweating and serum CGRP（r=-0.259,p=0.02）.The second experiment:Study on the difference of serum levels of FSH,LH,E₂,β-Ep and CGRP in patients with kidney Yang deficiency syndrome and kidney Yin deficiency syndrome of menopause syndrome（1）The general information:There was no significant difference in age distribution between kidney-yang deficiency group（45.93±2.98）and kidney-yin deficiency group（51.03±3.18）（p>0.05）.Among the enrolled patients,the top five symptoms of patients with kidney-Yang deficiency syndrome were chills of the extremities（93.33%）,forgetfulness（93.33%）,hypolibido（90.00%）,joint cold pain（80%）,and waist and abdomen cold pain（70%）.The top five symptoms in patients with kidney Yin deficiency syndrome were forgetfulness（96.67%）,irritability（80.00%）,insomnia（76.67%）,dry mouth and throat（70%）,and eye irritation（70%）.（2）Serum FSH,LH and E₂ levels of patients in kidney-yang deficiency group and kidney-yin deficiency group:The serum levels of FSH（69.070±40.473）and LH（27.379±14.616）in patients with kidney yang deficiency syndrome group were lower than those in kidney Yin deficiency group（72.260±35.118）and LH（33.465±19.130）,with no statistically significant difference（p>0.05）.There was no significant difference between serum E₂（34.523±18.909）in patients with kidney Yang deficiency group of menopause syndrome and E₂（34.620±13.926）in patients with kidney Yin deficiency group（p>0.05）.Through correlation analysis,it was found that there was no significant correlation between kidney Yang deficiency syndrome,kidney Yin deficiency syndrome and serum FSH,LH and E₂ in the menopausal syndrome（p>0.05）.（3）Levels of serumβ-Ep of patients in kidney-yang deficiency group andkidney-yin deficiency group:The serumβ-Ep of patients in the kidney Yang deficiency group of menopause syndrome（0.339±0.037）was higher than that in the kidney Yin deficiency group（0.336±0.030）,but the difference was not statistically significant（p>0.05）.The CGRP of kidney-yang deficiency group（0.499±0.046）was lower than that of kidney-yin deficiency group（0.503±0.048）,but the difference was not statistically significant（p>0.05）.Through correlation analysis,it was found that there was no significant correlation between the kidney Yang deficiency syndrome and kidney Yin deficiency syndrome of menopause syndrome and serumβ-Ep and CGRP（p>0.05）.Conclusion:The first experiment:FSH、LH、E₂:The serum levels of FSH and LH in perimenopausal women were significantly higher than those in childbearing age,while the levels of E₂ were significantly lower.Serum levels of FSH and LH in patients with menopausal syndrome were significantly higher than those in normal perimenopausal women,while E₂ levels were significantly lower.With the aggravation of the disease,serum FSH and E₂ levels in patients with menopausal syndrome showed an upward trend,with no statistically significant difference in LH.Serum FSH and E₂ increased with the aggravation of symptoms of hot flashes in patients with menopausal syndrome,but the difference was not statistically significant.The change in LH is not obvious.β-Ep、CGRP:The level of serumβ-Ep of women in the perimenopausal period decreased significantly compared with that in the childbearing age period,while the level of CGRP increased.Compared with normal women in perimenopausal period,the serumβ-Ep of patients with menopause syndrome decreased significantly,and the level of CGRP increased significantly.The levels of serumβ-Ep and CGRP in patients with menopause syndrome decreased with the aggravation of the disease degree,but the difference was not statistically significant.With the aggravation of hectic fever and sweating symptoms,the level of serumβ-Ep increased and the level of CGRP decreased,but the difference was not statistically significant.The correlation between serum CGRP level and the score of symptoms of hectic fever and sweating was significantly weak and negative.The second experiment:Serum levels of FSH,LH and E₂ in the menopausal syndrome kidney-yang deficiency group and kidney-Yin deficiency group were lower than those in kidney-Yin deficiency group,and serumβ-Ep levels in kidney-Yang deficiency group were lower than those in kidney-Yin deficiency group.Serum CGRP level in kidney-Yang deficiency group was higher than that in kidney-Yin deficiency group,but the difference was not statistically significant.