Effects Of Telomere-Binding Protein Gene Polymorphisms On Cholinesterase Activity In Omethoate-exposed Workers

Purpose:The aims of study were to learn the changes of cholinesterase activities and telomere lengths in workers and explore the effects of telomere binding protein gene polymorphisms on cholinesterase activities and telomere length,which was to screen susceptible population with decreased cholinesterase activities and genetic damage in omethoate-exposed workers.Subjects:A total of 180 workers in omethoate workshops of an agricultural factory were selected as the exposed group of the study,and 115 occupational health people were selected as the control group,which worked in the same city without a history exposed to organophosphorus pesticides and other poisons.After informed consent,questionnaire survey and biological sample were collected from subjects.Methods:The cholinesterase activities of whole blood,red blood cells and plasma in peripheral blood were determined according to the method for the determination of cholinesterase activities of whole blood,red blood cells and plasma in the appendix of Chinese occupational health standard GBZ 52-2002.Telomere length in peripheral blood leukocytes was determined by real-time quantitative polymerase chain reaction.The telomere-binding protein polymorphisms(TPP1 rs1800752,TPP1 rs3758978,TPP1 rs7488,TPP1 rs1128396,TPP1 rs2555173,POT1 rs17246404,POT1 rs7782410,POT1 rs13240512,and POT1 rs76436625)were genotyped with the Sequenom MassARRAY(?)matrix-assisted laser desorption/ionization-time of flight(MALDI-TOF)mass spectrometry platform(Sequenom Inc.,San Diego,CA,USA),and their genotypes were detected by a biotech company(Bio Miao Biological Technology Co.,Ltd.Beijing).Statistical analysis:The EpiData3.1 software was used to input data,and SPSS 21.0 software was used for statistical analysis.The chi-square test was used for qualitative data,and the appropriate analyze method was used for quantitative data according to the distribution of data.The covariance analysis was adopted to analyze the differences of distribution within the group.The generalized linear model was applied to analyze the combination and interaction effect of genes and omethoate on cholinesterase activities or telomere lengths,and screen the influencing factors of cholinesterase activity in whole blood or telomere length.All statistical tests were two-sided,and the level of statistical significance was set at a=0.05.Results:1.The factors of affecting the activities of whole blood cholinesterase:The results of single factor analysis showed that,individuals with CT+CC genotype,CC genotype,and CT genotype had lower whole blood cholinesterase activity than those with TT genotype in TPP1 rs 1800752 in the exposed group,respectively(P<0.05);In the exposed group,individuals with CG+GG genotype,GG genotype and CG genotype had lower whole blood cholinesterase activity than those with CC genotype in TPP1 rs3758978,respectively(P<0.10);In the exposed group,individuals with AT+TT genotype,TT genotype and AT genotype had lower whole blood cholinesterase activity than those with AA genotype in TPP1 rs1128396,respectively(P<0.10);In the exposed group,individuals with CC genotype and CC+AC genotype had lower whole blood cholinesterase activity than those with AA genotype in TPP1 rs2555173,respectively(P<0.05);In the exposed group,individuals with GG genotype had higher whole blood cholinesterase activity than those with AA+AG in POT1 rs7782410,while individuals with GG genotype had lower whole blood cholinesterase activity than those with AA+AG genotype in the control group.The results of the interaction analysis showed that the interaction of GG genotype in POT1 rs7782410,and AA genotype in POT1 rs13240512 and omethoate had an effect on the whole blood cholinesterase activity after adjusting for age,gender,smoking,and drinking,respectively[POT1 rs7782410:0.471(0.171,0.770),P=0.002;POT1 rs13240512:0.351(0.054,0.648),P=0.021].The multi-factor analysis by the generalized linear model showed omethoate[-1.604(-1.804,-1.405),P<0.001],CC+AC genotype in TPP1 rs2555173[0.289(0.005,0.573),P=0.046],and the interactions of omethoate and GG genotype in POT1 rs7782410[1.106(0.200,2.011),P=0.017]were the main influencing factors of the whole blood cholinesterase activity after adjusting for age,gender,smoking,and drinking.2.The factors of affecting telomere length:we did not found the effect of telomere binding protein gene polymorphisms on telomere length in single factor analysis and multi-factors screening.Conclusion:Omethoate,AA genotype in TPP1 rs2555173,and the interaction of AA+AG genotype in POT1 rs7782410 and omethoate were factors contributing to the decrease of the whole blood cholinesterase activity,suggesting that the individuals with AA genotype in TPP1 rs2555173 or AA+AG genotype in POT1 rs7782410 may be susceptible populations with lower whole blood cholinesterase activity in omethoate workers.