H₂S Improves Neurogenesis In The Hippocampal Of Diabetic Rats:Involves Activating Akt

[Objective]This work intends to explore whether Hydrogen sulfide（H2S）improve neurogenesis in the hippocampal of Streptozotocin（STZ）-induced diabetic rats,and whether this fuction involves activation of Akt.[Methods]1.Rats were intraperitoneally injected with STZ（55mg/kg）,and fasting blood glucose was more than 16.7mmol/L,which was considered as a successful diabetic model.2.SD rats were randomly divided into 5 groups:control group,STZ treatment group,STZ+low-concentration H2S（30 mol/kg）treatment group,STZ+high-concentration H2S（100 mol/kg）treatment group,and single high-concentration H2S（100 mol/kg）treatment group.Diabetic rats were given intraperitoneal injection of NaHS（30μmol/kg）and NaHS（100μmol/kg）once a day for 4 weeks.After the rats were killed,the hippocampal tissue was taken to detect the indicators.3.The expressions of Neurogenesis-associated proteins,including Doublecortin（DCX）,Glial fibrillary acidic protein（GFAP）,Neuron-specific nuclei protein（NeuN）;Akt signaling protein kinase B（AKT）and 473-serinephosphorylated kinase（p-AKT）in hippocampus were detected by western blot.[Results]1.H₂S improved hippocampal neurogenesis in STZ-induced diabetic ratsNeurogenesis-associated proteins DCX and NeuN decreased significantly and GFAP increased significantly in STZ-induced diabetic rats.After treatment with Sodium hydrosulfide（NaHS,a donor of H2S,100μmol/kg,i.p.）,the expressions of DCX、NeuN protein were increased significantly,the expression of GFAP protein was decreased significantly.These results showed that H₂S improved neurogenesis in the hippocampus of STZ-induced diabetic rats.2.H₂S activates Akt signaling in the hippocampus of STZ-induced diabetic ratsThe expression of AKT protein had no obvious effect and p-AKT protein decreased significantly in hippocampal of in STZ-induced diabetic rats.After NaHS treatment,there was no significant change in the expression of AKT,while the expression of p-AKT increased significantly.These results showed that H₂S improves neurogenesis in the hippocampus of STZ-induced diabetic rats involves the activation of Akt.[Conclusion]H₂S can improve neurogenesis in the hippocampus of STZ-induced diabetic rat,which is involved in the activation of Akt...