Preliminary Study On The Mechanism Of Mifepristone In Inhibiting The Proliferation And Invasion Of Epithelial Ovarian Cancer Cells

Objective: The purpose of this study was to investigate the affection of mifepristone to proliferation,invasion and migration of epithelial ovarian cancer.Investigate whether the mechanism of inhibiting cancer cell proliferation was related to the cell cycle and observe whether the regulatory effect of mifepristone was related to drug concentration.Consstructed a mouse model of ovarian cancer subcutaneous transplantation tumor,and observe the inhibitory effect of different doses of mifepristone on ovarian cancer transplantation tumor.Method:1.The effects of different concentrations(25μmol/L,50μmol/L,75μmol/L,75μmol/L,100μmol/L)of mifepristone on the proliferation,invasion and migration of epithelial ovarian cancer ID8 cell were detected by MTT assay and Transwell experiments.2.Flow cytometry was used to detect whether mifepristone affected the proliferation of ovarian cancer by affecting the cell cycle.3.C57BL-6 mice were selected to construct a mouse model of subcutaneous transplantation tumor with ovarian cancer ID8 cells,which were randomly divided into a control group(oral double-distilled water)and low,medium and high dose mifepristone groups(mifepristone0.5mg/kg/d,10mg/kg/d,30mg/kg/d,intragastric administration),loplatin group(5mg/kg,intraperitoneal injection),coffee group.The effect of mifepristone on the growth of transplanted ovarian cancer was observed by measuring the volume of the transplanted tumor every day and drawing a tumor growth curve.The expression of Ki67 and CD34 in the transplanted tumors of mice were detected by immunohistochemistry.Results:1.As the concentration of mifepristone increases,the proliferation inhibition rate of ID8 cells gradually increases,and the number of migrating and invading cells decreases,suggesting that mifepristone inhibits the proliferation,migration,and invasion of ID8 cells in a concentration-dependent manner.2.Flow cytometry showed that the number of cells blocked in the G1 phase of the mifepristone experimental group was higher than that of the control group,and the number of cells entering the S phase was smaller than that of the control group.3.Animal experiment show that the volume growth rate of ovarian cancer xenograft mice in the high-dose group of mifepristone is lower than that in the control group,and the expression of Ki67 and CD34 in the xenograft tissue is lower than that in the control group.Conclusions:Mifepristone may inhibit the proliferation,invasion and migration of epithelial ovarian cancer ID8 cells by affecting the cell cycle and angiogenesis.