| 1.Aim To observe and analyze the effects of moxibustion on serum levels of interleukin(IL)-1β,IL-6,tumor necrosis factor(TNF)-α,and the Toll-like receptor 4(TLR 4),nuclearfactorkappa B(NF-κ B)protein and m RNA expression in colon tissue of rats with diarrheal irritable bowel syndrome(IBS-D)by intragastric administration of Senna leaves combined with rough cotton and hemp rope.To study the mechanism of moxibustion at Tianshu(ST25)and Shangjuxu(ST37)in the treatment of IBS-D.2.Methods40 SD rats were randomly divided into 4 groups: blank group(n = 10),model group(n= 10),inhibitor group(n = 10)and moxibustion group(n = 10).Except the blank group,the rats in the three groups except the blank group were fed with Senna leaf immersion solution combined with rough cotton rope to bind both hind limbs to replicate the IBS-D model.After the replication of the model was completed,the inhibitor group was given intraperitoneal injection of TLR 4 inhibitor TAK 242 with a dose of0.3mg/100g/day,once a day.The moxibustion group was treated with mild moxibustion at Tianshu and Shangjuxu acupoints for 30min/d,and both groups were treated for 7 days.In the course of the experiment,the general state,the rate of defecation and the minimum volume threshold of abdominal retraction reflex were observed and measured in each group.After the treatment,the rats were anesthetized,the blood of the abdominal aorta was drawn,the colonic tissue was collected,and finally the rats were killed.Colonic tissue sections were stained with HE.The contents of IL-1β,IL-6 and TNF-α in serum of rats in each group were detected by ELISA method.The expression levels of TLR 4 and NF-κ B(p65)m RNA in colonic tissue of each group were detected by RT-PCR method.The expression levels of TLR 4 and NF-κ B(p65)protein in colonic tissue of each group were detected by Westernblot method.The experimental data are processed and statistically analyzed by SPSS21.0 software.3.Results1)The general state of rats in each group: during the whole experiment,the rats in the blank group ate normally,their hair color was white and shiny,the action was agile,and there was little fighting phenomenon.After the establishment of the model,the defecation times of the other three groups increased,the stool became thinner,the coat color withered,the weight lost,and the phenomenon of biting and fighting often occurred.After treatment,the diet,coat color and defecation of rats in the inhibitor group and moxibustion group were improved.2)The loose stool rate and the minimum volume threshold of abdominal retraction reflex in each group: compared with the blank group,the loose stool rate in the model group increased(P< 0.05),and the minimum volume threshold of abdominal retraction reflex decreased(P< 0.05).Compared with the model group,the diarrhea rate in the inhibitor group and moxibustion group decreased(P< 0.05),the minimum volume threshold of abdominal retraction reflex increased(P< 0.05),and the minimum volume threshold of abdominal retraction reflex in the moxibustion group was significantly lower than that in the inhibitor group(P< 0.05).The minimum volume threshold of abdominal retraction reflex in the moxibustion group was significantly higher than that in the inhibitor group(P< 0.05).3)HE staining of colonic tissue of rats in each group: the magnification was 200 times.There were no pathological changes and inflammatory infiltration in the blank group,mild inflammatory reaction,no obvious erosion and ulcer in the colonic mucosa of the model group,and no obvious pathological changes and neutrophil infiltration in the moxibustion group and the inhibitor group.4)The levels of IL-1β,IL-6 and TNF-α in serum of rats in each group: compared with the blank group,the levels of IL-1β,IL-6 and TNF-α in serum of model group were all increased.Compared with the model group,the serum levels of IL-1β,IL-6 and TNF-α in the inhibitor group and moxibustion group decreased.The serum levels of IL-1β,IL-6 and TNF-α in the moxibustion group were lower than those in the inhibitor group.5)The expression of TLR 4 and NF-kappa B(p65)protein and their m RNA in colon tissue: compared with the blank group,the expression of TLR 4 and NF-kappa B(p65)protein and m RNA in colon tissue of model group increased(P< 0 05).Compared with the model group,the expressions of TLR 4,NF-kappa B(p65)protein and m RNA in colon tissue in inhibitor group and moxibustion group were decreased(P< 0.05).There was no significant difference in TLR4 Protein and m RNA expression between moxibustion group and inhibitor group,but the expression of NF-kappa B(p65)protein and m RNA in the colon tissue of the moxibustion group was lower than that of the inhibitor group(P< 0.05).4.Conclusions1)Moxibustion at Tianshu and Shangjuxu acupoints can improve the general condition,diarrhea,visceral hypersensitivity and low-grade inflammation of colonic mucosa in IBS-D rats.2)Moxibustion may improve the symptoms of IBS-D rats by inhibiting downstream IL-1β,IL-6 and TNF-α through TLR 4/NF-κ B pathway. |
PDF Full Text Request