Effects Of ZMS On Learning And Memory Of D-Galactose-Induced Aging Rats

Objective: To observe the effects of sapogenin from zhimu(ZMS)on the learning and memory functions of aging rats induced by D-galactose.Methods: Thirty-two male SD rats were randomly divided into 4 groups(n = 8): normal control group(intraperitoneal injection of normal saline once a day,qd,for 6 weeks),aging model group(intraperitoneal injection of 1% D-galactose 0.5 ml/100 g,qd,for 6 consecutive weeks),ZMS low-dose group(intraperitoneal injection of 1% D-galactose 0.5ml/100 g to prepare the model,while intragastric administration of ZMS,3.6 mg/kg,equivalent to 1/5 of the recommended clinical dose of human,qd,for 6 consecutive weeks),ZMS high-dose group(intraperitoneal injection of 1% D-galactose 0.5 ml/100 g to prepare the model,while intragastric administration of ZMS,18 mg / kg,equivalent to the recommended clinical dose of human,qd,for 6 consecutive weeks).Six-day water maze test was used to detect the learning and memory functions of rats since the next day after the end of treatment.The water maze experiment is divided into three parts,including the,location navigation experiment and spatial probe trial The escape latency in place navigation trials,the first time to reach the platform,the number of cross platform and the ratio of target quadrant activity time in spatial probe trial were recorded.Results:(1)location navigation experiment: the escape latency of aging model group was longer than that of normal control group(P < 0.05);the escape latency of ZMS low-dose group was longer than that of high-dose group(P <0.01);the escape latency of ZMS high-dose and low-dose group was shorter than that of model group(P < 0.01).On the 1st,2nd and 5th day of the location navigation experimentt,the escape latency of ZMS high dose group was shorter than that of the normal control group(P < 0.01),and on the 4th and 6th day of location navigation experiment,the escape latency of ZMS low dose group was shorter than that of the normal control group(P < 0.05).(2)spatial probe trial:(1)The first time to reach the platform in the aging model group and ZMS high dose group was significantly longer than that in the normal control group(P <0.01),and that in ZMS high and low dose group was shorter than that in the aging model group(P < 0.01).(2)The target quadrant activity time of aging model group had shorter tendency as compared with that of normal control group(P = 0.06),and ZMS low dose group was longer than that of aging model(P < 0.05).(3)The number of cross platform in ZMS low dose group was more than that in aging model group(P = 0.05),and that in ZMS high dose group was more than that in aging model group(P < 0.05).Conclusion: ZMS can dose-dependently prevent the decline of learning and memory functions of aging rats induced by D-galactose to some extent.