Study On The Effect Of Lijie Capsule In Treating The Mice With Collagen-induced Arthritis From Intestinal Flora

Objective: To observe the changes of intestinal flora structure of collagen-induced arthritis mice and the effect of Lijie Capsule on it,explore the mechanism of Lijie Capsule treatment of RA from the perspective of intestinal microecology.Methods: Sixty male C57BL/6 mice were divided into three groups by random number table: normal group、model group、Lijie capsule group and20 per group,except for the normal group,the model The CIA model was induced in both groups and Lijie capsule group.The CIA mice in the Lijie Capsule group were administered intragastrically with Lijie Capsules daily,and the normal group and the model group were treated with equal amounts of sterilized double distilled water for 21 days.The toe joint swelling degree and arthritis index score(AI)were used as the evaluation criteria for the efficacy;16Sr DNA sequencing technology was used to compare and analyze the characteristics of the intestinal flora of each group of mice.First analyze the differences in α diversity of the intestinal flora of each group of mice,including differences in operating taxonomic unit(OTU),species abundance index(Chao,Ace),and diversity index(Shanno,Simpson);then analyze the βdiversity differences Finally,the differences in intestinal species composition of mice in each group were statistically analyzed.Results:(1)Weight: Compared with the normal group,the weight of the mice in the model group and the Lijie capsule group was reduced after modeling(P<0.05).Compared with the model group,the mice in the Lijie capsule groupgained weight after 2 weeks of administration(P<0.05).(2)Toe swelling:Compared with the normal group,the toe swelling of mice in each group were significantly increased after modeling(P<0.01).Compared with the model group,the toes swelling of mice in the Lijie capsule group reduced after 2weeks of administration(P<0.05),and the reduction was significant after 3weeks(P<0.01).(3)AI value: The AI index of model mice were significantly different from the normal group(P<0.01).Compared with the model group,the AI value of mice in the Lijie capsule group were different after 2 weeks of administration(P<0.05),and the difference is significantly after 3 weeks of administration(P<0.01).(4)Alpha diversity analysis: diversity of model mice decreased: compared with the normal group,the Shannon index of the model group decreased and Simpson index increased(P<0.05).Compared with the model group,the Shannon index of Lijie capsule group increased and Simpson index decreased(P<0.05).(5)β diversity analysis: The unweighted distance matrix based on Uni Frac suggests that the intestinal flora of the three groups of mice were significantly different(P<0.01).(6)Species composition:Compared with the normal group,the rising bacterial genus in the model group is Streptococcus(P<0.05),the bacterial genus that has risen significantly are Desulfovibrio and Clostridium Xl Va(P<0.01).After recepted Lijie capsule treatment,the Streptococcus and Clostridium Xl Va decreased significantly(P<0.01),and the levels of Desulfovibrio were also reduced(P<0.05).Compared with the normal group,there are five kinds of bacterial genus in the model group decreased(P<0.05),namely Lactobacillus,Barnesiella,Allobaculum,Bacteroide and Erysipelotrichace,while the level of Bifidobacterium decreased significantly(P<0.01).Compared with the model group,the levels of Barnesiella 、 Allobaculum and Erysipelotrichace in Lijie Capsule group increased(P<0.05).The level ofBifidobacterium increased significantly(P<0.01).However,the level of Lactobacillus had no differences(P>0.05).Conclusions: The intestinal flora diversity of modeling mice decreased,and the component of bacterial flora changed significantly.The most obvious ones were the increase of Desulfovibrio,Clostridium Xl Va and the decline of Bifidobacterium.Lijie capsule can increase the diversity of intestinal flora of CIA mice and improve the uniformity of the flora.Especially,it can inhibit the growth of Streptococcus for the diseased mice,and have a significant effect of supporting the growth of Bifidobacterium.This study confirmed that Lijie Capsules can relieve arthritis and control the progression of RA by adjusting the structure and composition of intestinal flora in CIA mice.