Study On Serum Lipid Level And Variations Of Intestinal Flora In Breast Cancer Patients And Their Correlations

Objetive:To analyze the serum lipid levels between the breast cancer patients and the healthy person,we compare the differences of two groups and further explore the relationship between the occurrence of breast cancer and its pathological characterist-cs with serum lipid level.At the same time,using quantitative real-time PCR,we sought to compare the quantity difference among Eubacterium rectal,Bacteroides multiline and Bifidobacterium in breast cancer patients and the healthy person.At last to explore the correlation between the serum lipid level and the content of intestinal flora.To seek the risk factors of breast cancer.Provide new ideas for the prevention and adjuvant treatment of breast cancer.Methods:We gathered 160 cases of patients with invasive breast cancer who underw-ent breast surgery in our hospital from December 2018 to December 2019,160 cases of healthy women who were tested in our department during the same period as control group.We collected the clinical data and recorded the serum lipid levels of two groups.To analyze the relationship between lipid metabolism and the risk of breast cancer.A subgroup analysis of patients in breast cancer group was conducted to explore the relationship between serum lipid levels and tumor size,lymphnode meta-stasis and tissue.The collected data were analyzed using statistical software SPSS 23.0 and the difference of serum lipid level was examined by t-test.Fecal samples were collected and extracted by DNA extraction kit.According to the test taret-speci-fic primers 16S rRNA bacterial gene sequences,using extracted genome DNA as template,amplified by quantitative real-time PCR reaction.When the reaction was completed,we calculated the fecal sample copy number bacterial gene,as the quanta-tive results.The data were analyzed using statistical software SPSS 23.0 and the difference of intestinal flora was examined by t-test.Pearson correlation analysis was used to analyze the relationship of lipid metabolism index and genes copy number in Eubacterium rectal,Bacteroides multiline and Bifidobacteriumin in patients with breast cancer.With P<0.05 was considered statistically significant.Results:(1)Serum TC,TG and LDL-C expression were obviously higher in the patie-nts with breast cancer than those in the control group,serum HDL-C expression was obviously lower than the control group.(2)Serum TC and TG expression were obviously higher in the premenopausal patients with breast cancer than those in the premenopausal subjects of control group,serum HDL-C expression was obviously lower than the control group.And serum TC and LDL-C expression were obviously higher in the postmenopausal patients with breast cancer than those of postmenopaus-al subjects in control group,serum HDL-C expression was obviously lower than the control group.(3)Analysis of the relationship between serum lipid level and pathological characteristics of breast cancer patients,it was found that serum TC and LDL-C expression were higher in breast patients of stage ?+? than those stage ?+?.The relationship between the serum lipid level and other pathological of breast cancer was not found.(4)The content of Eubacterium rectal and Bifidobacterium were lower in breast cancer patients than those in the control group,the content of Bacteroides multiline was no significant difference in two groups.(5)Pearson correlation analysis showed the content of Bacteroides multiline was positively correlated with the level of serun TC.And the content of Bifidobacterium genes were negatively correlated with the levels of TC and LDL-C in postmenopausal patients with breast cancer.Conclusion:(1)Serum lipid level abnormality and intestinal flora disorder may be related to the occurrence of breast cancer.(2)The increase of serum TC and LDL-C and the decrease of Bifidobacterium genes may become the risk factors of breast cancer.