Ginsenoside Rg1 Alleviates Hepatic Ischemia Reperfusion Injury In Rats By Regulating Endoplasmic Reticulum Stress

Objective:To observe the effect of ginsenoside Rg1(G-Rg1)on hepatic ischemia reperfusion injury(HIRI)in rats and further explore its mechanism.Methods:SD rats were randomly divided into sham surgery(S)group,ischemia reperfusion(I/R)group,and G-Rg1 treatment(G)group.In the I/R group,a model of partial(70%)heat ischemia-reperfusion injury in the liver of rats was established.In the G group,G-Rg1 20mg/kg·d was given two days before the operation in two intraperitoneal injections.And then collect serum and liver tissues of each group at 1h,6h and 24h postoperatively,and groupes were further divided into S1、S6、S24,I/R1、I/R6、I/R24,G1、G6、G24 according to the collection time,and 7 per group.Then detection serum alanine aminotransferase(ALT)and radix asparagi aminotransferase(AST),HE staining to evaluate liver tissue pathology change,TUNEL method to detect liver tissue apoptosis,quantitative PCR method to detect the rat the endoplasmic reticulum stress related protein protein 78(GRP78),C/EBP homology protein(CHOP)mRNA,Western Bloting to detect the contents of protein GRP78 and CHOP.Results:The rat liver ischemia reperfusion model was successfully established Compared with the S group,serum ALT and AST concentrations in the I/R group increased significantly(P<0.01),liver tissue damage was serious,and apoptosis rate increased(P<0.05).GRP78 expression was time-dependent.Changes in mRNA and protein levels were consistent,increased at 1h(P<0.05),decreased at 6h(P<0.05),increased at 24h(P<0.05),and CHOP only increased at 6h(P<0.05).Compared with the I/R group,ALT and AST were significantly reduced in G group(P<0.01),tissue damage was reduced,and apoptotic cells were reduced(P<0.05).GRP78 protein content increased at all three time points(P<0.05),while CHOP protein content decreased at all three time points(P<0.05).Conclusions:1.The model of hot ischemia-reperfusion injury in rat liver(70%)is a stable,reliable,simple and operable model.2.G-rgl pretreatment can protect rat liver and reduce hepatocyte apoptosis induced by ischemia-reperfusion.3.Endoplasmic reticulum stress is involved in hepatic ischemia-reperfusion injury and regulates the recovery of liver function in a time-dependent manner;4.Ginsenoside Rg1 can reduce hepatic ischemia reperfusion injury in rats,and its mechanism may be related to regulation of endoplasmic reticulum stress.5.The PERK-CHOP pathway of ERS unfolded protein response plays a role in the early stage of hepatic ischemia-reperfusion injury.