| Background and objectiveGastric cancer,which is a malignant tumor originating from the Gastric mucosal epithelium,is the fourth most common cancer in the world and the second most deadly cancer.The statistics in 2015 showed that the number of patients with newly developed gastric cancer in China was about 679,000,and the disease mortality rate was as high as 73.34%.Among them,the metastasis of gastric cancer was one of the main causes of death.However,the early symptoms of gastric cancer are not specific,and most patients are already in the advanced stage or have metastasis when diagnosed,which misses the best opportunity for surgical treatment.To search for biomarkers that can be used in the early diagnosis of gastric cancer,to judge the risk of metastasis and predict the prognosis of patients,has great clinical significance for the early diagnosis and treatment of gastric cancer and the improvement of postoperative survival rate of gastric cancer.Long non-coding RNA(lncRNA)plays an important regulatory role in physiological activity.Previous studies have shown that lncRNA is closely related to the occurrence,progression and metastasis of a variety of tumors by interacting with miRNA,and can be used as a biomarker for early diagnosis and prognosis of tumors.In this study,we found the expression of LncRNA C21orF96 was up-regulated in gastric cancer lymph nodes and we use bioinformatics analysis to analyze the interaction sites between LncRNA C21orF96 and miR-875-5p,while miR-875-5p acted on the 3 ’UTR region of USF2 gene.Therefore,the purpose of this study was to investigate the role of LncRNA C21orF96 in gastric cancer cells and its mechanism.Methods1.Extract total RNA of gastric cancer lymph node metastases cell line SGC-7901,KATO Ⅲ and distant metastases gastric cancer cell line SNU-16,after analysis of purity,reverse transcription for cDNA.Use the real-time quantitative reverse transcription-polymerase chain reaction to test the expression of micro RNA related to the lncRNA-C21orF96 in the gastric cancer cell line2.Plasmid was used to over-express the expression of lncRNA C21orF96 in KATO-Ⅲ and siRNA was used to knockdown the expression of LncRNA C21orF96 in SGC-7901,and RT-PCR was used to measure the expression of LncRNA C21orF96,miRNA-875-5p and USF2 genes3.After the overexpression of LncRNA C21orF96 in MKN45,transwell was used to observe changes in the ability of cells to invade and migrate.Results1.LncRNA C21orF96 showed a significant inverse relationship with miR-875-5p,and the relative quantitative values of the two were statistically different2.In KATO-Ⅲ which over-express the expression of LncRNA C21orF96,miR-875-5p expression decreased significantly while USF2 expression increased;In SGC-7901 which knockdown the expression of LncRNA C21orF96,miR-875-5p expression increased significantly while USF2 expression decreased3.In the experiments to detect the invasion and migration ability of gastric cancer cells,the average number of cells passing through the artificial basement membrane in the experimental group after overexpression of LncRNA C21orF96 was significantly different from that in the control group(migration:216.19±2.30 vs 89.19 ± 4.60,p<0.001;invasion:146.18 ± 5.3 vs 59.18±2.60,p<0.001)Conclusions1.In some gastric cancer cell lines,the expression of LncRNA C21orF96 can reduce the expression of miR-875-5p and promote the expression of USF2.2.In gastric cancer cells,LncRNA C21orF96 may influence tumor invasion and migration through its ability to regulate miR-875-5p and USF2. |
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