Study On The Changes Of Intestinal Flora In NAFLD Combined With Abnormal Glucose Metabolism

Objective:1.The serum-related indicators of rats in the high-fat diet group and the normal diet group were detected,and the expression levels of Eubacterium rectum,Bacteroides polymorpha,Lactobacillus,and Bifidobacterium in the intestines of the rats were detected by qRT-PCR.The pathological changes of liver and ileal tissues in the two groups were observed,so as to explore the role of the enteric-liver axis in the occurrence and development of non-alcoholic fatty liver disease.2.The expression levels of Eubacterium rectum,Bacteroi des polymorpha,Lactobacillus,and Bifidobacterium in the intestines of patients with NAFLD complicated with abnormal glucose metabolism were detected to investigate the changes of intestinal microflora.Methods:1.20 healthy male SD rats were randomly divided into normal feed group(n=10)and high-fat diet group(n=10).2.After 9 weeks of feeding,the basic indicators such as body weight,liver wet weight,blood sugar,blood lipids,liver function and other basic indicators were detected.qRT-PCR was used to detect the expression of the above target flora in the rat intestine.HE and oil red staining were used to observe the pathological changes of the liver and ileum tissue.The NAFLD activity score was calculated based on the liver staining results.3.A total of 131 NAFLD patients with abnormal glucose met abolism were selected as the experimental group,while 66 healthy subjects were selected as the normal control group.Clinical data of the subjects were collected,and the intestinal target bacteria expression was detected by qRT-PCR.Results:1.The serum TG and GLU levels of rats in the high-fat diet group were significantly higher than those in the normal diet group(P＜0.05).The levels of AST,ALT and LDL-C in the high-fat diet group were higher than those in the normal diet group.While LDL-C was lower than that in the normal diet group,but the difference was not statistically significant(P＞0.05).2.Compared with the general diet group,the body mass,liver wet weight and liver wet weight/body weight of rats in the high-fat diet group were signi ficantly increased(P＜0.05).HE staining of liver tissues in the high-fat diet group showed extensive vacuolar degeneration of liver tissues,large amount of lipid droplets deposition,and inflammatory cell infiltration in liver lobules.Oil red staining showed that lipid droplets in hepatocytes were stained with dark red.HE staining of ileum showed that the intestinal villi became blunt and more in testinal villi fused with each other.3.Compared with the normal diet group,the NAFLD activity score of the high-fat diet group was significantly increased,and the difference was statistically significant(P＜0.05).4.Compared with the normal diet group,the expression levels of Lactobacillus and Bacteroides polyformis in the intestinal tract of the rats in the high-fat diet group were significantly decreased(P＜0.05).There were no statistically significant differences between the two groups in the expressions of Eukaryotes and Bifidobacterium(P＞0.05).5.Compared with the normal control group,the age,BMI,FPG、TC、TG,LDL-C,ALT,γ-GGT,HbAlc,and 2hPG in the NAFLD with abnormal glucose metabolism group were significantly increased(P＜0.05).6.The expression levels of Eubacterium rectum and Lactobacillus in NAFLD with abnormal glucose metabolism group were significantly higher than that of normal control group(P＜0.05).The expression level of Bifidobacterium was significantly lower than that of normal control group(P＜0.05).There was no statistically significant difference in the expression of multiple lines of Bacteroides between the two groups(P＞0.05).Conclusion:1.Compared with the normal diet group,the the rats in the high-fat diet group had abnormal glucose and lipid metabolism,and extensive fatty changes could be seen in liver tissues.This method could be used to explore the mechanism of metabolism-related diseases and establish an ideal animal model for drug experiments.2.Compared with the normal diet group,the ileal mucosa of the rats in the high-fat diet group was damaged,and the expression levels of intestinal lactobacilli and Bacteroides polymorpha were significantly reduced。Suggesting that high-fat diets may have intestinal flora disorders and intestinal barrier pathological changes.3.Compared with the normal control group,these results may show that NAFLD patients with abnormal glucose metabolism were significantly obese or overweight,which was closely related to metabolic disorders.4.Compared with the normal control group,the expressions of etobacter rectum and lactobacillus in the intestinal tract of patients with NAFLD complicated with glucose metabolism were significantly increased,while the expressions of bifidobacteria were significantly decreased.These may suggest that patients with NAFLD complicated with glucose metabolism might have changes in intestinal flora structure.