| Objective: This study intends to use obese mouse models constructed from high-fat diet mice as research objects.By observing the effects of ketogenic diet(KD)feeding on obesity mice’s weight,serum and liver lipid metabolism-related indexes,we explored the effects of KD on obese mice’s weight and liver lipid metabolism.Methods: A mouse model of obesity was constructed by feeding high-fat diet.Then,obese mice were randomly divided into a ketogenic diet group(KD),a high-fat diet group(HFD),and an obese control group(SD).They were fed a ketogenic diet,a high-fat diet,and a standard diet.The control group at that time was set as one group as the standard control group(CON);the body weight of the mice was recorded every seven days and the change trend was observed.When feeding in groups for 2 weeks,half of the mice in each group were randomly selected and anesthetized and sacrificed,and serum,liver and other tissues of the mice were taken.After group feeding for 8 weeks,the remaining mice were killed and harvested.q RT-PCR was used to detect the m RNA expression of Apo B100、Cd36、Ppar-α、Cpt-1a、Fgf21 and Cyp2e1 in mouse liver.ELISA kits were used to detect Apo B100,TNF-α,IL-6,IL-8 and HNE levels in mouse liver.Western Blot was used to detect the expression levels of CD36,PPAR-α,CPT-1A,FGF21 and CYP2E1 in mouse liver.Results:(1)After 10 weeks of high-fat feeding,the average weight of mice in the obese model group increased by 23.1% compared with the control group.(t = 4.1059,P = 0.001),the model was successfully created.After grouping,the average weight of the mice in KD,HFD and SD groups was similar,and the weight of the three groups was higher than that of the CON group(P <0.01).(2)After the group intervention,the weight of mice in the HFD group showed an upward trend.After 8 weeks of group intervention,the weight of the mice in the HFD group was much higher than other groups,while the weight of the mice in the KD and SD groups decreased.No difference from the CON group.(3)At 2 weeks of group intervention,the TG concentration in the serum of the KD group mice increased abnormally(P <0.05);at 8 weeks of intervention,the TG in the serum of the KD group mice decreased(P <0.05).During 2 weeks of KD intervention,the levels of AST and ALT in serum of mice in KD group increased abnormally(P <0.05),but the levels of serum AST and ALT in mice of KD group decreased significantly after 8 weeks of intervention(P <0.05).(4)At 2 weeks of KD intervention,liver TG levels in the KD group were significantly higher than those in the other three groups(P <0.05).After 6 weeks of continued intervention,liver TG levels in the KD group significantly decreased(P <0.05).There was no significant difference in liver TG levels between SD and CON mice.(5)Regardless of 2 or 8 weeks of intervention,the expression levels of Apo B100,CD36,PPARα,FGF21,and CYP2E1 in the KD group and the HFD group were significantly higher than those in the SD and CON groups(P <0.01).(6)At 2 weeks of KD intervention,liver TNF-α levels in the KD group were higher than those in the other three groups(P <0.005).After 8 weeks of intervention,the levels of TNF-α,IL-6 and IL-8 in the KD group were all higher.Decreased to a certain extent(P <0.05).Conclusion: From the outcome of the study,KD can help mice lose weight and improve liver fat accumulation;however,it should be noted that the liver fat accumulation and liver injury in mice at the early stage of KD intervention are worse,and 8 After one week,the liver fat content of the mice in the KD group is still significantly higher than that in the standard diet intervention mice.Therefore,from the liver perspective,it may be feasible to use KD to reduce weight in obese people,but it should be in the condition of good liver function.Application of KD,and it is not recommended to use KD for a long time.After the weight reaches normal standards or after one year of application,the diet pattern should be adjusted in time to prevent irreversible damage to the liver. |
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