Effect Of Ferroptosis In Peritoneal Fibrosis Rats

Objective:The purpose of this study was to investigate the expression of ferroptosis in peritoneal fibrosis rats,and to provide new ideas for the prevention and treatment of peritoneal fibrosis.Methods:Twenty male SD rats weighing 200±10g were purchased and adaptively bred for 3 days under constant temperature（22±2）℃,constant humidity（55±5）%,standard light,free diet,and drinking water.Three days later,they were randomly divided into four groups A,B,C,and D.The test lasted 4 weeks.Group A was a control group:received no treatment;Group B was a model group:treated with daily intraperitoneal injection of 4.25%peritoneal dialysate(100 mL·kg^-1body weight);Group C was a solvent group:treated with daily intraperitoneal injection of DMSO,after 1 hour,treated with daily intraperitoneal injection of 4.25%peritoneal dialysate(100 mL·kg^-1body weight);Group D was a intervention group:treated with daily intraperitoneal injection of Fer-1,after 1 hour,treated with daily intraperitoneal injection of 4.25%peritoneal dialysate(100mL·kg^-1body weight).After 4 weeks,the rat peritoneal tissue samples were retained,some of them were fixed in 10%formalin neutral buffer for subsequent section staining tests,and the rest were stored at-80℃for subsequent Western blot and Real-time quantitative PCR（qRT-PCR）detection.HE staining and Masson staining were used to observe the structure of rats peritoneal tissues,and the expressions of FN,E-cad,GPX4 and SLC7A11 were detected by real-time quantitative PCR and Western blot.By measuring the above indicators,the mRNA and protein expression levels of of FN,E-cad,GPX4 and SLC7A11 were compared between groups A and B,the mRNA expression levels of of FN,E-cad were compared between group B and D、group B and C.Results:1.Compared with group A,group B showed thickened peritoneal tissues,round mesothelial cells,increased collagen fibers,a large number of inflammatory cells and neovascularization.The mRNA and protein expression of FN in group B was higher than that in group A,The mRNA and protein expression of E-cad、GPX4 and SLC7A11 in group B was lower than that in group A.2.Group A and B showed thickened peritoneal tissues,round mesothelial cells,increased collagen fibers,a large number of inflammatory cells and neovascularization.3.Compared with group B,The mRNA expression of FN in group D was decreased and the mRNA expression of E-cad was increased.Conclusion:1.Peritoneal fibrosis rats can be successfully constructed by using 4.25%peritoneal dialysate.2.The key regulatory genes for ferroptosis,such as GPX4 and SLC7A11 were expressed in peritoneal fibrosis rats.3.The use of ferroptosis inhibitor Fer-1 can inhibit the progress of peritoneal fibrosis rats,suggesting that ferroptosis has a potential role in peritoneal fibrosis rats.Intervention of ferroptosis may provide new treatments for peritoneal fibrosis.