Evaluation Of The Expression Of Matrix Metalloproteinase-1 Of Laryngeal Squamous Cell Carcinoma By Ultrasound Molecular Imaging

Purpose:The aims of this study were to evaluate the expression of Matrix Metalloproteinase-1(MMP-1)on Laryngeal Squamous Cell Carcinoma(LSCC).Methods:The microsized MMP-1-targeted microbubbles(MBMMP-i)and the control MB s(MBigG)based on perfluorocarbon-filled lipid-shelled MBs were constructed and characterized.The in vitro binding experiment was performed with human epidermoid laryngeal cancer cells(HEp-2)and tested the binding efficiency of MBMMP-i and MBigG.In the in vivo study,the LSCC model was established in 10 mice.The MBMMP-1 and MBigG were randomly injected into tumor-bearing mice via the tail vein at Day 7,Day 12,and Day 17 to dynamically evaluate the differential targeted enhancement(dTE)signals via USMI.Subsequent immunofluorescence analysis was used for confirmation of MMP-1 expression.Result:The effective adhesion rate of MBMMP-1 and MBIgG to HEp-2 was 298.42±16.57 versus 12.38±3.26 bubbles/per field in vitro experiment,which shows a significant difference(P<0.01).The in vivo USMI results demonstrated that dTE signal intensity from MBMMP-1 was significantly higher than that from the MBIgG at Day 7,Day 12,and Day 17(Day 7,41.21±15.00 versus 2.25±0.6 a.u.,P<0.05;Day 12,124.64±5.19 versus 11.13±1.13 a.u.,P<0.05;Day 17,332.01±64.88 versus 42.99±11.9 a.u.,P<0.01).Moreover,immunofluorescence analysis further confirmed the expression of MMP-1 in LSCC with a gradual increase with the tumor growth.Conclusion:MBMMP-1 could be a ultrasound molecular imaging probe that can be used in the early diagnosis of LSCC by USMI.