The Interaction Between TERT-CLPTM1L Gene Polymorphism And Smoking Exposure On The Incidence Of Nasopharyngeal Carcinoma

Objective: Nasopharyngeal carcinoma(NPC)is a common origin of nasopharyngeal mucosa epithelial cells of malignant tumor,has obvious racial predisposition and familial aggregation phenomenon.Families and races with a high incidence of the disease still have a high incidence of the disease in their offspring after emigration(or emigration).Worldwide,nasopharyngeal cancer accounts for 0.6% of all malignancies,with approximately 71% of new cases in southeast,central Asia and northern Africa.Therefore,based on the known environmental risk factors(smoking,drinking,preserved food,betel nut,oral hygiene),it is particularly important to further study the role of genetic mutations in the development of nasopharyngeal carcinoma in the field of molecular genetics.TERT-CLPTM1 L is a kind of is located in the tumor susceptibility of target gene on the chromosome 5p15.33,the polymorphisms of change impact telomerasereverse transcriptase to adjust the length of telomeres at the end of the genetic bases,thus affecting tumor genesis and development.In recent years,with the continuous development and maturity of the new generation of gene sequencing technology,single nucleotide polymorphisms(SNPs)of TERT-CLPTM1 L gene have been proved to affect the occurrence and development of a variety of tumors.It is provided theoretical basis that the detection of oncogenes in nasopharyngeal carcinoma and the early screening of susceptible population to study the differential expression of different TERT-CLPTM1 L genotypes between nasopharyngeal carcinoma patients and normal population.This study provided a theoretical basis for early screening of high-risk population and exploration of new tumor biomarkers through stratification analysis and the study of the interaction between genes and environmental factors through single nucleotide polymorphism of TERT-CLPTM1 L gene on susceptibility to nasopharyngeal cancer.Methods: This study use the case-control research method.The case sample selection in The first affiliated hospital of China medical university otolaryngology department outpatient service of nasopharyngeal pathology and obtain definite pathological diagnosis of 200 cases of patients,according to age(+/-5 years of age)over the same frequency group matching,to select in the center of the medical examination of healthy examination people as control.Venous blood and epidemiological information were collected from all subjects.This study was approved by the ethics committee of China medical university,and the subjects signed the informed consent for epidemiological data collection and blood sample collection and abided by the relevant regulations of ethics.In this study,DNA in white blood cells was extracted by the classical phenolic chlorine method,and the DNA was amplified by Taqman real-time PCR.Genotyping of the rs2736100 and rs401681 sites of TERT-CLPTM1 L was performed.SPSS22.0software was used for data processing and statistical analysis.All the statistical tests were double-sided tests,and P<0.05 was taken as the criterion of statistical significance.The x2 test was used to compare the distribution of demographic characteristics,exposure to related risk factors,and SNP genotypes between the case group and the control group.Non-conditional Logistic regression model was used to calculate OR and 95% CI to analyze the relationship between polymorphism changes of TERT-CLPTM1 L genotypesand the risk of nasopharyngeal carcinoma.Results: TERT-CLPTM1 L genetically rs2736100 and two rs401681 polymorphism generally has nothing to do with the genetic susceptibility to nasopharyngeal carcinoma(NPC),but by gender stratification found that type rs401681 carry mutations in male patients with nasopharyngeal carcinoma CT+CC maybe associated with a higher risk of NPC,the P value is 0.010,OR values were 2.139 and 95%CI=1.199-3.818.The rs2736100 has not been found to be associated with nasopharyngeal cancer in men.In addition,in the overall population study,it was found that the smoking rate of nasopharyngeal cancer patients was higher than that of normal healthy people(P<0.001).Further analysis of the relationship between the proportion of wild type allele T in rs401681 and smoking found that there was an interaction between the two.Conclusion: 1.Two mutated genotypes of TERT-CLPTM1 L gene rs401681 CT+CC may increase the risk of nasopharyngeal squamous cell carcinoma in male patients.2.Smoking is a risk factor for nasopharyngeal carcinoma and interacts with allele C.