Association Between Serum Circ-0054633 And Insulin Resistance In Patients With Type 2 Diabetes Mellitus

Background:Diabetes is a worldwide epidemic of metabolic disorders^[1],and type 2diabetes（T2DM）accounts for 90-95%of all diabetes^[2].The pathogenesis of T2DM is not well understood,but insulin resistance（IR）is widely believed to be the main pathogenesis.Studying the molecular mechanism of IR can provide a new target for the diagnosis and treatment of T2DM.Zhao Z et al collected circRNA from the peripheral blood of 6 healthy controls and 6 T2DM patients for microarray analysis and conducted an independent cohort study of 20 normal cases,20 patients with abnormal glucose tolerance and 20 T2DM patients.In the T2DM group,a total of 489 circRNA were found to be differentially expressed between the two groups,78 upregulated and 411downregulated,the largest area under the circ-0054633 curve,suggesting that peripheral blood circ-0054633 are significantly expressed in T2DM patients^[3].Recently,it has been found that circ-0054633 has a certain diagnostic value for pre-diabetes and T2DM,and has the potential to be used as a diagnostic marker for both plasma^[4].Objective:Serum circ-0054633 level of T2DM patients was studied in this experiment,its relationship with glycolipid metabolism and IR was discussed,provide further theoretical basis for clinical study of the pathogenesis of diabetes.Methods:Peripheral blood samples were collected from 30 patients with T2DM and 32patients with nondiabetic disease,centrifugal,kit was used to extract RNA,ultraviolet spectral index RNA concentration and purity,inspection and reverse transcription after using quantitative reverse transcription polymerase chain reaction（qRT-PCR）technique,to validate the circ-0054633 study,to explore whether the circ-0054633 differentially expressed correlation with IR.Results:1.Compared with the normal group,there were statistically significant differences in fasting blood-glucose（FPG）,2 hours postprandial blood sugar（2hBG）,glycosylated hemoglobin(HbA_1C),homeostatic model assessment for Insulin resistance index（HOMA-IR）,triglyceride（TG）and high-density lipoprotein（HDL-C）in the diabetic group（P<0.05）.2.The expression level of serum circ-0054633 in the diabetic patients was significantly higher than the control group（P<0.05）,with statistically significant differences.3.Correlation analysis found that the expression level of serum circ-0054633was significantly positively correlated with HbA_1C,TG and HOMA-IR（r=0.358,0.286,0.434,P<0.05）,and the expression level of serum circ-0054633 was negatively correlated with HDL-C（r=-0.165,P<0.05）.4.Multiple linear regression analysis was conducted with serum circ-0054633 as the dependent variable and HDL-C,TG,FPG,2hBG,HbA_1C,HOMA-IR and serum circ-0054633 as independent variables.The results showed that FPG,HOMA-IR and TG were independently correlated with serum circ-0054633（P<0.05）.Conclution:The level of serum circ-0054633 expression in patients with T2DM is increased,which may be closely related to glucose and lipid metabolism disorder and IR.