Protective Effect Of Biochanin A On Cerebral Ischemia-reperfusion Damage Mice And Dementia Mice

Objective:In this paper,we investigated the neuroprotective effect of biochanin A(Bio A)on cerebral ischemia-reperfusion damage mice and D-galactose combined with scopolamine hydrobromide-induced dementia mice,and explored its neuroprotective mechanism.MethodsThe experiment was divided into two stages: the first stage was the model test of cerebral ischemia-reperfusion damage mice,and the second stage was the dementia model test induced by D-galactose combined with scopoletin hydrobromide.In the first stage,the model of cerebral ischemia-reperfusion damage mice was established by bilateral common carotid artery occlusion(BCCAo).The changes of learning and memory in sham group,model group and Bio A treatment groups(5 mg/kg,10 mg/kg and20 mg/kg)were evaluated by Y maze test.The activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)and the content of malondialdehyde(MDA)in cortex and hippocampus of mice were determined using the kits.Moreover,the structural changes of neuronal cells in hippocampal CA1 region of mice were observed by HE staining.In the second stage,the aging of mice was induced by D-galactose continuous subcutaneous injection of the neck and intraperitoneal injection of scopolamine hydrobromide.The positive group and treated groups were given β-estradiol(0.35 mg/kg)and Bio A group(5 mg/kg,10 mg/kg and 20 mg/kg)by gavage,and the other groups were given normal saline.The Y-maze test was used for the learning and memory test,and then we used the kits to determine the antioxidant index(SOD,GSH-Px activity and MDA content)in the cortex and hippocampus of mice.HE staining was used to observe the morphological changes of neurons in hippocampal CA1 region of dementia mice.ResultsIn the first stage,the results of Y maze test showed that the learning and memory ability of the mice treated with high dose of Bio A(20 mg/kg)was significantly improved,and the activities of SOD and GSH-Px in the cerebral cortex of the mice were significantly increased.The content of MDA in the cortex of each treatment group was significantly lower than that of the model group.The pathological sections stained with HE showed that the neurons in CA1 region of hippocampus in the Bio A treatment groups were repaired and positive dose correlations were present.In the second stage,Y-maze test showed that the correct times of learning and memory in Bio A treatment groups and positive control group were higher than those in model group.Bio A can increase theactivity of SOD and GSH-Px in cortex and hippocampus,and decrease the content of MDA.The pathological sections stained with HE under the 200× microscope showed that Bio A could repair the injured neurons in the CA1 region of the hippocampus of mice.All the results showed a positive dose correlation.ConclusionBio A can play an antioxidant role in the brain area of mice with cerebral ischemia-reperfusion injury,scavenge free radicals,balance the oxidation and antioxidant processes,protect the cells in the hippocampal CA1 region of mice,and improve their learning and memory abilities.In addition,Bio A can also increase the activity of SOD and GSH-Px in the cortex and hippocampus of D-galactose combined with scopolamine hydrobromide-induced dementia mice,and reduce MDA content in injured mouse cortex and hippocampus.The structure of the nerve cells has a certain repairing effect,improving the learning and memory ability of the dementia mice.