| Objective: To explore the role and possible mechanism of Salvianic acid a sodium in improving the motion ability of rats with spinal cord injury,and to provide a theoretical basis for the study of spinal cord injury treatment.Methods: Ninety healthy SD rats were randomly divided into three groups: blank control,model control and Salvianic acid a sodium group,30 rats in each group.Modified Allen’s method was used to perform T10 spinal cord injury model construction for model control group and Salvianic acid a sodium group.After successful modeling,rats in the Salvianic acid a sodium were injected with 1ml /kg of Salvianic acid a sodium intraperitoneally daily.The rats in the blank control group and the model control group were injected intraperitoneally with the same amount of normal saline,which last for 21 days.At 1,3,7,14 and 21 days after the intervention,the motor function of each group of rats was assessed using the BBB scoring method;Blood was collected from the tail vein of the rats,and interleukin 8(IL-8)and interleukin-1β(IL-1β)levels were determined by ELISA assay in each group.;Rats in each group were sacrificed on days 3 and 21 days,and spinal cord tissues were taken for paraffin embedding,dehydration,slide preparation,and HE staining.The morphological changes of spinal cord tissue were observed in each group;on day 21,the levels of PI3 K,Akt,and mTOR mRNA level in the spinal cord tissue of three groups of rats were detected by real-time quantitative fluorescence PCR(RT-qPCR);SPSS Pearson correlation analysis software was used to determine correlation between motion recovery after spinal cord injury and PI3 K,Akt,and mTOR mRNA levels.Results: On the 1 and 3 days after intervention,there was no statistical difference between the motion function scores of the rats in sodium danshensu treatment group and the model control group(P> 0.05).On the 7,14,and 21 days after the intervention,the motion function score of the rats in the Salvianic acid a sodium treatment group was significantly higher than that in the model control group(P <0.05).After 3 days of intervention,in model control group,patchy hemorrhage was seen under the spinal tissue microscope,the number of nerve cells was relatively small,cell infiltration was observed;after 21 days of intervention,the spinal cord tissue was severely loose,forming a large number of vacuoles,and there were a large number of inflammatory cell infiltrations;after 3 days of intervention in the Salvianic acid a sodium group,spinal cord injury was obvious,and a few tissue vacuoles were visible;after 21 days of intervention,tissue vacuoles relatively few,the inflammatory cells and glial cells were significantly reduced;compared with the model control group,the levels of inflammatory factors IL-8 and IL-1β in Salvianic acid a sodium intervention group were significantly reduced on the 1,3,7,14 and 21 days after the intervention(P <0.05),but the levels of inflammatory factors IL-8 and IL-1β in the Salvianic acid a sodium group were not statistically different at different time points(1,3,7,14,and 21 days)(P> 0.05);PI3K,Akt,and mTOR mRNA level was higher in Salvianic acid a sodium group than that of the model control group(P <0.05)and lower than that of the blank control group(P <0.05),and they were positively correlated with motion ability recovery in spinal cord injury rats(P <0.05).Conclusion: Salvianic acid a sodium can improve motion ability of spinal cord injured rats,release inflammatory response,and reduce inflammatory factor levels.The levels of PI3 K,Akt and mTOR mRNA were positively correlated with the recovery of motion capacity in spinal cord injured rats. |
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