Preparation Of Magnetic Ganoderma Lucidum Spore Microspheres And Its Application

Ganoderma lucidum spore(GLS)is the generative cells at late growth stage of the sporophore for Ganoderma lucidum,which belongs to natural macromolecule herbal medicine in traditional Chinese medicine.It has become a very promising natural micro-carriers in the drug delivery for potential application due to its large interior space,thermal stability,non-toxicity and uniformity.In this thesis,GLS was used as the raw material,which was magnetized by chemical coprecipitation,and the prepared magnetic Ganoderma lucidum spore(MGLS)was applied to the study of targeted drug delivery and dye adsorption.Firstly,the natural GLS was treated by chemical and physical methods,and the experiments were optimized based on the results by scanning electron microscopy(SEM).After treatment with acetone degreasing,potassium hydroxide solution and orthophosphoric acid solution,the pores on the surface of the GLS widened.From the SEM image,it was observed that the inner wall was flat and smooth,and the channel was formed from internal to external.Then,Fe3O4 nanoparticles were deposited by chemical coprecipitation method,and the prepared MGLS was characterized and analyzed by SEM,EDS,XRD,VSM and FTIR.Next the release process was explored in vitro for the MGLS loaded with Rhodamine B(RhB),the results showed that the cumulative release reached 47.3%,88.4% and 92.3% at 6,24 and 48 hours,respectively.The results indicated that the MGLS has a significant effect on the sustained release for the RhB.Secondly,the MGLS was used as the drug delivery carrier and loading the anti-tumor drug 5-Fluorouracil(5-FU),and characterized by SEM,EDS,XRD,VSM,FTIR and UV-Vis.We found that the maximum of the loaded 5-FU reached 250.23mg/g for MGLS in the initial concentration of 5-FU solution 5 mg/mL.After 48 hours,the cumulative release of the assembly 5-FU@MGLS reached 80.11% and 67.14% in PBS and HCl solutions,respectively.The effect of 5-FU@MGLS on the proliferation of SGC-7901 cells in vitro was examined at the cellular level,and the MGLS as a sustained release drug carrier were further verified.Thirdly,the 5-FU@MGLS was applied to tumor-bearing mice,and the5-FU@MGLS was aggregated at the tumor site under the action of an external magnetic field,showing good targeting.By measuring the blood drug concentration at different times and the distribution of drug absorption in various tissues,it wasverified that the 5-FU@MGLS has a significant effect for targeted release on mice.The results showed that MGLS had potential application in targeted drug delivery.Finally,MGLS was used as adsorbent to remove the refractory dyes Rhodamine B(RhB)and methyl orange(MO),and the effect of the different adsorption conditions on the adsorption performance was explored.The regeneration ability of MGLS was investigated,and the adsorbed MGLS was desorbed with ethanol and then used to adsorb the corresponding dyes.The results showed that MGLS had a high recycling rate.