Association Between RGS6 Gene Polymorphism And Lung Cancer Risk In Northeast Chinese Han Population

Purpose: Lung cancer,the most common malignant tumor,is the leading cause of cancer deaths.Studies have shown that individual genetic susceptibility plays an indispensable role in the occurrence and development of lung cancer.Singe necleotide polymorphism(SNP)is the most common genetic variation in the human genome,and a large number of epidemiological studies have shown that changes in SNP can affect tumor formation,invasion,metastasis and prognosis.Regulator of G-protein Signaling 6(RGS6)is down-regulated in many human malignancies and plays an important role in energy metabolism,apoptosis and tumorigenesis.Recent studies suggest that RGS6 might be involved in tumorigenesis of lung cancer.However,limited knowledge is known about RGS6 in lung cancer.This is a case–control study which designed to demonstrate the individual or interaction of two SNPs(rs12885387and rs2283381)in the RGS6 gene with the risk of lung cancer,as well as the gene–environment interaction between the polymorphisms and tobacco exposure in our Northeastern Han population.Material and methods: This study including 683 cases and 802 controls,it was a hospital-based case-control study.Two SNP loci rs12885387 and rs2283381 in the RGS6 gene were selected,and genotyped by Taqman probe fluorescence quantitative PCR allele identification technology.All statistical analyses were performed using IBM SPSS Statistics 22.Student’s t test was used to calculate continuous variables;The gender,age,smoking status and histological type of the case group and the control group were compared by chi-square test;Non-conditional logistic regression analysis calculates odds ratios and 95% confidence intervals to assess the relationship between genetic polymorphisms and susceptibility to lung cancer;Crossover analysis was used to evaluate the interaction between SNPs and environmental exposure;The multiplicative and additive models were used to verify the relationship between gene-environment interactions and lung cancer risk.Results: Both rs12885387 and rs2283381 polymorphisms were found to be associated with lung cancer risk after adjusting for gender,age,smoking and other factors.In rs12885387,compared with those carrying homozygous AA genotypes’ people,carriers of CC genotype(adjusted OR=2.485,95%CI=1.723-3.584,P<0.001)have an increased risk of lung cancer;Carriers of TT genotype in rs12885387(adjusted OR=2.301,95%CI=1.560-3.394,P<0.001)had an increased lung cancer risk compared with CC genotype carriers.Stratification of lung cancer histological types revealed that both genotypes were statistically significant in adenocarcinoma and squamous cell carcinoma(P<0.05).The results of crossover analysis demonstrated that there was significant interaction between rs12885387,rs2283381 and tobacco exposure(P<0.05),but there was no statistically significant interaction in the additive and multiplicative models.Conclusion: The RGS6(rs12885387 and rs2283381)polymorphism may be statistically related to the risk of lung cancer and its pathological subtypes in Northeast Chinese Han population.