The Association Between The Polymorphism And Placental Expression Of MOV10 With Preeclampsia

Objective Preeclampsia is a pregnancy-specific disorder,which often leads to multiple organ and system damage and seriously threatens the health of mothers and fetus.Genetic factor is considering to contributing to the pathogenesis of preeclampsia.Single nucleotide polymorphisms(SNPs)of several genes were found to have relationship with preeclampsia,especially hypertension-related genes.The aim of this study was to investigate the association between rs2932538 polymorphism of MOV10 and susceptibility to preeclampsia,and to evaluate whether the expression level of MOV10 is altered in PE.Methods 1531 normal pregnant women and 956 preeclamptic patients were admitted by the Affiliated Hospitals of Qingdao University,Shandong Provincial Hospital and Binzhou Medical University Hospital From January 2016 to January 2018.The clinical data and test results of the subjects were collected,and the total genomic DNA was extracted.The rs2932538 polymorphism of MOV10 was genotyped by TaqMan probe fluorescence quantitative PCR(qPCR),besides,the differences of clinical data,genotype distribution and allele frequencis between these two groups were analyzed.The placental tissues of 25 healthy pregnant women and 25 preeclamptic patients were collected by the Affiliated Hospital of Qingdao University from July 2017 to January 2018.The total RNA and protein of placenta were extracted.qPCR was used to assess the expression level of MOV10 mRNA in cases and controls,furthermore,the expression level of MOV10 protein was detected by Western blot.Results(1)There was no significant difference on age between the case group and the control group(P > 0.05).The gestational age and the neonatal weight of cases were significantly lower than controls(P < 0.001).When compared to the controls,the cases has significantly higher gestational weight gain,systolic pressure,diastolic pressure,triglyceride,total cholesterol,ALT,AST,creatinine and blood urea nitrogen(P < 0.001).(2)There are significant differences between preeclamptic patients and healthy subjects on the genotype distribution and allele frequencies(P < 0.001).(3)The carriers of T alleles have higher risk of preeclampsia than the carriers of C alleles(P < 0.001,OR = 1.569,95% CI = 1.339-1.838).(4)The genotype distribution and allele frequency of mild/severe preeclampsia group were significantly different from the control group(mild preeclampsia vs.control: genotype P = 0.014;allele P = 0.012,OR = 1.522,95% CI = 1.094-2.116;severe preeclampsia vs.control: genotype P<0.001;allele P < 0.001,OR = 1.577,95% CI = 1.336-1.860).The genotype distribution and allele frequency of the early /late onset preeclampsia group were statistically different when compared with controls,respectively(early onset preeclampsia vs.control group: genotype P < 0.001,allele P < 0.001,OR = 1.580,95% CI = 1.311-1.904;late onset preeclampsia vs.control: genotype P < 0.001,allele P < 0.001,OR = 1.554,95% CI = 1.260-1.917).However,no statistical difference were found on genotype and allele frequency between mild preeclampsia and severe preeclampsia,early onset preeclampsia and late onset preeclampsia(P > 0.05);(5)The placental expression level of MOV10 mRNA and protein in case group were found significantly lower than control group(P < 0.05).Conclusion(1)The rs2932538 polymorphism of MOV10 may be related to the susceptibility to preeclampsia in the Chinese Han population,however,no association was found bewtween polymorphism of rs2932538 and severity or onset time of preeclampsia.(2)The low level expression of MOV10 in placenta of preeclampsia may be closely related to the pathogenesis of preeclampsia.