Effects Of Hesperidin On Retinal Ganglion Cells In Diabetic Rats And Its Mechanism

Objectives To investigate whether hesperidin has anti-apoptotic effect on retinal ganglion cells in diabetic rats and its mechanism.Methods There were 36 male SD rats,with no obvious abnormality in the anterior segment and fundus of the eyes,randomly divided into normal control group(C group),diabetic injury group(D group),hesperidin treatment group(H group).Every group all have 12 rats.D group and H group establish diabetic rat model by injecting streptozotocin(STZ).C group was injected with 0.01 mol/L sodium citrate buffer at the same dose.After the diabetes model was established successfully and the blood glucose was stable,H group was treated with hesperidin(50mg/kg)intragastric administration daily for 8 weeks,and the C group and the D group were given equal volume saline intragastric administration everyday for 8 weeks.The general condition of rats was observed every day,including: hair color,activity,appetite,drinking water,etc.Fasting blood glucose was measured and recorded once a week.After 8 weeks,all animals were killed,the right eye was quickly taken to prepare paraffin sections,and the left eye was quickly taken placed in liquid nitrogen.HE staining was used to observe retinal morphology.Tunel staining was used to observe neuron apoptosis in retina and count the apoptosis index(AI).The expression of caspase-3 protein was measured by immunohistochemistry.The Real-time Quantitative PCR Detecting System(qPCR)was used to detected the relative expression of brain-derived neurotrophic factor(BDNF)and tyrosine protein kinase receptor B(TrkB).Results 1 Blood glucose at the same time: D group and H group was higher than that of the C group,and the blood glucose of the H group was lower than that of the D group,and the difference was statistically significant(P<0.05).It is confirmed that hesperidin is hypoglycemic.2 HE: Rats in group C had distinct retinal layers,regular arrangement and good ganglion cell morphology.The retinal of rats in group.D group of rats had loose and thin retinal tissue,obvious cell edema,nuclear contraction and crescent nucleus,the arrangement of cells was disordered,and the number of ganglion cells decreased significantly.The pathological changes in the H group were lighter than those in the D group,the cell edema was relatively lighter,the arrangement was relatively regular,and the number of ganglion cells was slightly reduced.Its confirmed that hesperidin reduced retinal damage in diabetic rats.3 Tunel and apoptosis index: Apoptotic cells were observed in the retinal tissues of the three groups.The apoptosis index(AI)of D group and were higher than that of C group,AI of H group was lower than that of D group.The difference of AI between the three groups was significant(P<0.05).Its confirmed that hesperidin can inhibits apoptosis4 of RGCs.4 caspase-3 protein expression level: caspase-3 protein was observed in all three groups.The average optical density value(AOD)of rats in the three groups was different.The AOD value of the D group was higher than that of the C group,and the AOD value of the H group was lower than that of the D group,the three groups was statistically significant(p<0.05).Its confirmed that hesperidin decreased the expression of caspase-3.5 The relative expression of BDNF and TrkBmRNA BDNFmRNA in group D was lower than that in group C,and BDNFmRNA in group H was higher than that in group D,and the difference between the three groups was statistically significant(P<0.05).The content of TrkBmRNA in D group was lower than that in C group,and TrkBmRNA in group H was higher than that in group D,the difference was statistically significant(P<0.05).Its confirmed that hesperidin increased the expression of BDNF and TrkB.Conclusions 1 Hesperidin inhibits RGCs apoptosis in diabetic rats.2 Anapoptotic effect of hesperidin is related to its hypoglycemic effect and BDNF / TrkB signaling pathway.Figure8;Table11;Reference 134...