The Effect Of Interaction Between High Temperature Operation And RAAS Gene Polymorphism On The Essential Hypertension In An Iron And Steel Enterprise Workers

Objective The onset of essential hypertension(EH)is determined jointly by genetic and environmental factors.Among genetic factors,the renin-angiotensin-aldosterone system(RAAS)plays an important role in the pathogenesis of EH,high temperature is an occupational risk factor for EH,RAAS and high temperature may have an interactive effect on EH.A case-control study was conducted to explore the effect of the interaction between RAAS gene polymorphisms and high-temperature exposure on the susceptibility of steel workers to EH.Methods A case-control study was conducted on 793 male workers from a steel company,including 284 in the EH group and 509 in the control group.Occupational epidemiological survey was conducted on the study subjects,and venous blood was collected.High temperature and noise exposure of the workers were detected.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was used to detect polymorphisms in RAAS gene loci.The Hardy-Weinberg equilibrium(HWE)test was performed on the control group.In the association analysis of RAAS gene polymorphism and EH,the allele and genotype distribution of each RAAS gene locus in EH group and control group were compared.Unconditional Logistic regression,bifurcation method and generalized multifactor dimensionality reduction(GMDR)were used to analyze the effect of the interaction between genetic polymorphisms and high-temperature operations on the susceptibility of male steel workers to EH.Results 1 The high temperature exposure ratio of the EH group was higher than that of the control group,the difference was statistically significant(P<0.05).2 Analysis of the association between RAAS gene polymorphisms and EH were analyzed.The results showed that the G allele at the A-6G locus of the AGT gene was 1.487 times more likely to have EH than the A allele(95%CI: 1.222-1.804),the risk of EH for GG genotype was3.018 times that of AA genotype(95%CI: 2.022-4.505);the C allele in the CYP11B2gene-344T/C locus was 1.303 times more likely to have EH than the T allele(95%CI:1.044-1.629),the CC genotype was 2.028 times more likely to have EH than the TT genotype(95%CI: 1.199-3.429).3 Multi-factor Logistic regression analysis on the impact of the environment and genes on EH showed that the risk of EH in high-temperature workers was 1.523 times that of non-high-temperature workers(95%CI: 1.096-2.117);at the A-6G locus of the AGT gene,carrying the GG genotype was 2.805 times more likely to develop EH than the AA genotype(95%CI: 1.808-4.351);at the CYP11B2 gene-344T/C locus,carring the CC genotype was 2.027 times more likely to have EH than the TT genotype(95%CI: 1.115-3.685).4 The results of gene-gene interaction analysis based on the multiplication model show that the AGT gene A-6G site and the CYP11B2 gene-344T/C site had phase-based interactions.Compared with carrying AA genotype of AGT gene A-6G locus and the TT genotype of the CYP11B2-344T/C locus,the OR to be EH of carrying GG genotype of AGT gene A-6G locus and the CC genotype of the CYP11B2-344T/C locus was 4.183(95%CI: 1.485-11.781);the result of the interaction analysis of the gene-high temperature multiplication model shows that compared to carrying the AA genotype of AGT A-6G who were not exposed to high temperatures,the OR of EH of carrying the GG genotype of AGT A-6G and exposed to high temperatures was 10.884(95%CI: 4.610-25.700).5 In the analysis of GMDR interaction,the interaction between RAAS gene polymorphisms and high temperature operation is the best model,workers who were homozygous for mutants and exposed to high temperature at the same time were 3.221 times more likely to have EH than workers who were homozygous for wild type and exposed to no high temperature(95%CI: 1.992-5.208).Conclusions 1 High temperature will increase the risk of EH;AGT gene A-6G locus gene polymorphism GG genotype,CYP11B2 gene-344T/C locus gene polymorphism CC genotype can increase the susceptibility to EH.2 The AGT gene A-6G locus gene polymorphism,respectively,and the CYP11B2 gene-344T/C locus and the presence of high temperature operations have a multiplicative interaction on the effect of EH,increasing the risk of EH.Figure 3;Table 21;Reference 162...