Screening Of Biomarkers In Serum Exosomes Of Patients With Epilepsy Based On Proteomics Technology

Epilepsy is one of the most common chronic neurological diseases in the world,high incidence,high risk of sudden unexplained death in epilepsy(SUDEP),difficult diagnosis.Exosomes are nanosized extracellular vesicles released into physical environments and carry a variety of biological information.Moreover,exosomes can also be synthesised and released from brain cells,passing through the blood-brain barrier(BBB),and can be detected in peripheral blood or cerebrospinal fluid.Our study,using tandem mass tag(TMT)showed that a total of 76 proteins were differentially expressed in serum exosomes between epilepsy patients and healthy controls,6 increasing and 70 decreasing.The results of the study data indicate that two significantly differentially expressed serum exosomal proteins F9 and TSP-1 serve as promising biomarkers for the diagnosis of epilepsy.This is the first study of serum exosomal proteins in epilepsy which suggests that exosomes are promising as "new tools" for the diagnosis of epilepsy.