Expression And Significance Of TIPE2 And FOXP3 In Liver Tissue Of Patients With Chronic Hepatitis,Cirrhosis And Hepatocellular Carcinoma

Objective: To analyze the expression levels of TIPE2 and FOXP3 in liver tissue of patients with chronic hepatitis,cirrhosis and hepatocellular carcinoma,and to explore the role of TIPE2 and FOXP3 in the pathogenesis of liver disease and the progression of the disease.Methods:1.Human subjectsSixty cases of paraffin-embedded liver tissue were collected from the Department of Traditional and Western Medical Hepatology and Hepatobiliary Surgery of the Third Hospital of Hebei Medical University in 2003-2018.The diagnosis was in accordance with China’s 2015 guidelines for the prevention and treatment of chronic hepatitis B,2015 guidelines for the prevention and treatment of chronic hepatitis C,2011 guidelines for the diagnosis and treatment of liver cirrhosis,2017 guidelines for the treatment of primary liver cancer and 2000 criteria for the prevention and treatment of viral hepatitis,while excluding other viral hepatitis overlapping infections and other serious diseases of the liver or other systems.2.Research methodsClinical data,laboratory data and liver histopathology were collected retrospectively to analyze and compare the differences between the groups.we collected archived paraffin-embedded normal liver tissues,chronic hepatitis,cirrhosis,hepatocellular carcinoma and paracancerous tissues.Immunohistochemical methods were used to detect the expression of TIPE2 and FOXP3 proteins in all liver tissues.Test or nonparametric rank sum test was used to compare the differences between groups,pearson to analyze the correlation between TIPE2 and FOXP3 and clinical indicators.Results:1.The expression of TIPE2 in liver tissue of patients with chronic hepatitis and hepatocellular carcinoma was lower than that of healthy control group(P<0.05),while FOXP3 expression was higher than that of healthy control group(P<0.05).2.The expression of TIPE2 was significantly lower in both ALT and AST abnormal groups than that in normal groups,while FOXP3 was higher in ALT abnormal groups,AST abnormal groups and normal groups,respectively(P<0.05).3.The clinicopathological analysis showed that the lower the degree of differentiation of cancer tissue,the lower the level of TIPE2 expression and the higher the FOXP3 expression;there was no statistical difference in TIPE2 and FOXP3 expression in cancer tissue.4.TIPE2 expression was negatively correlated with hepatocyte injury index ALT and AST levels,with peripheral blood N(neutrophils)and NLR(neutrophil-to-lymphocyte ratio)levels;TIPE2 negatively correlated with FOXP3 expression,respectively(P<0.05).Conclusions:1.The expression of TIPE2 in liver tissue of patients with chronic hepatitis and hepatocellular carcinoma was lower than that of healthy control group,while FOXP3 expression was higher than that of healthy control group.2.The level of TIPE2 expression was related to the degree of hepatocellular injury and the pathological grade of hepatocellular carcinoma;TIPE2 expression level of TIPE2 in cancer tissue was lower than that of paracancerous,but there was no statistical significance,and the consideration was related to the small number of samples.3.TIPE2 protein expression level was negatively correlated with hepatocyte injury index ALT,AST,peripheral blood index NLR and FOXP3 expression level.4.Above results suggest that TIPE2 may participate in the progression of liver disease through negative regulation of immune response and play different biological roles in different disease states.