Expression Level Of GPR120 MRNA And Influencing Factors In Omental Adipose Tissue Of Different Populations

ObjectiveThe purpose of this study was to investigate the expression of G protein coupled receptor 120(G protein-coupled receptor,GPR120)mRNA in omental adipose tissue of obese and Type 2 diabetic(Type 2 diabetesmellitus,T2DM)patients,and to explore its related factors.MethodsFrom December 2018 to December 2019,29 patients with normal weight(control group,18.5kg/m~2≤24kg/m~2),26 overweight patients(24kg/m~2≤28kg/m~2)and 17 obese patients(BMI≥28kg/m~2)were enrolled in the Department of General surgery of the first affiliated Hospital of Xi’an Medical College.At the same time,12 T2DM patients and 15 non-diabetic patients who underwent laparoscopic cholecystectomy in the first affiliated Hospital of Xi’an Medical College from December 2018 to December 2019 were collected.Height,weight,blood pressure,abdominal color ultrasound,blood glucose,blood lipid,liver function and kidney function of all patients were measured and collected,and Body mass index(BMI)and Waist-to-hip ratio(WHR)were calculated.Real-time fluorescence quantitative PCR was used to detect the expression of GPR120mRNA in omental adipose tissue,and one-way ANOVA,T-test,partial correlation analysis and multiple stepwise regression analysis were used for statistical analysis.Results1 Expression of GPR120mRNA and its influencing factors in omental adipose tissue of obese people1.1Comparison of general and biochemical data:There were significant differences in body weight,BMI,Waist circumference(WC),Hip circumference(HC),WHR,Lymphocyte(LY),Apolipoprotein E(Apo-E),Apolipoprotein a(Apo-a)and Uric acid(UA)among obesity group,overweight group and control grou p(P<0.05).However,there were differences in Triglyceride(TG),High densit-y lipoprotein-cholesterol(HDL-C),Apolipoprotein A1(Apo-A1),Blood Ureanitr ogen(BUN)and Serum creatinin(SCr),But the difference was not statistical ly significant(P>0.05).1.2The expression level of GPR120mRNA in obesity group and overweight greater omental adipose tissue was higher than that in normal weight control group(P<0.05),and the expression level of GPR120mRNA in greater omental adipose tissue in obesity group was higher than that in overweight group(P<0.05).1.3According to gender grouping,there were 25 males and 47 females.No matter male or female,the expression level of GPR120mRNA in omental adipose tissue of obesity group was significantly higher than that of overweight group and control group(P<0.05).1.4According to the results of abdominal ultrasound,all subjects were divided into two groups:fatty liver group and non-fatty liver group.The results showed that the expression level of GPR120mRNA in greater omentum adipose tissue of subjects with fatty liver was higher than that of non-fatty liver group,and the difference was statistically significant(P<0.05).1.5The correlation analysis between GPR120mRNA and clinical indexes showed that there was a positive correlation between GPR120mRNA and body weight(r=0.591),BMI(r=0.589),WC(r=0.448),HC(r=0.535),WHR(r=0.369)and TG(r=0.248).It was negatively correlated with HDL-C,Apo-A1 and BUN(P<0.05).1.6Multiple stepwise regression analysis was carried out with GPR120mRNA expression as dependent variable and age,BMI,WHR,blood glucose,TC,TG,HDL-C and LDL-C as independent variables.The results showed th-at BMI,Apo-A1 and WHR were independent related factors of GPR120mRNA expression level.2 Expression level and influencing factors of GPR120mRNA in greater omental adipose tissue in patients with diabetes mellitu.2.1Comparison of general and biochemical data:there were differences in age,WC,WHR,hypersensitive C-reactive protein,Fasting plasma glucose(F PG),alkaline phosphatase and Glomerular filtration rate(GFR)between diab-etic group and non-diabetic group,The difference was statistically significa-nt(P<0.05).2.2The expression of GPR120mRNA in omental adipose tissue of diabe-tic group was higher than that of non-diabetic group,The difference was statistically significant(P<0.05).2.3According to sex,there were 8 males and 19 females.The expressio-n level of GPR120mRNA in omental adipose tissue of males and females in diabetic group was higher than that in non-diabetic group,and there was statistical significance(P<0.05).2.4The diabetic group was divided into two groups according to the 5year course of disease.There was no significant difference in GPR120mRNA between the diabetic group and the control group(P>0.05).2.5According to the results of abdominal ultrasound,the patients were divided into two groups:fatty liver group and non-fatty liver group.The expression level of GPR120mRNA in omental adipose tissue of fatty liver group was higher than that of non-fatty liver group,but there was nostatistical significance(P>0.05).2.6The correlation between GPR120mRNA and clinical indexes suggeste-d that GPR120mRNA was positively correlated with age(r=0.401),WC(r=0.530),WHR(r=0.620),FPG(r=0.755),DBIL(r=0.561)and IBIL(r=0.391)(P<0.05).2.7Multiple stepwise regression analysis was conducted with the expression of GPR120mRNA in greater omental adipose tissue as dependent variables and age,BMI,WHR,blood glucose,TC,TG,HDL-C,LDL-C,Apo-A1 as independent variables.The results showed that blood glucose and Apo-B entered the model and were independent risk factors for GPR120mRNA expression in greater omental adipose tissue.Conclusions1The expression of GPR120mRNA in omental adipose tissue of obese patients was significantly increased,and BMI,Apo-A1 and WHR were the influencing factors of its expression.2The expression of GPR120mRNA in omental adipose tissue of patients with type 2 diabetes mellitus was significantly increased,and blood glucose and Apo-B were the influencing factors.Therefore,GPR120 may bea potential target for the treatment of metabolic diseases such as obesity and T2DM.