Experimental Study On The Effect Of Glacier Quick-acting Jiuxin Dropping Pills On Promoting Blood Circulation And Removing Stasis

Objective: In this study,the effect of "Bingchuansuxiaojiuxindiwan" on thrombosis was observed by rat external arteriovenous bypass thrombosis model,and the blood stasis syndrome model was used to observe the effect of "Bingchuansuxiaojiuxindiwan" on hemorheology in rats.To explore the related mechanism of glacial quick-acting and saving heart pills for promoting blood circulation and removing blood stasis.Methods: 1.Rat arteriovenous bypass thrombus model replication method,the experimental animal is stripped of the neck artery and vein(the common carotid artery and external jugular vein)under anesthesia,and a polyethylene tube with a silk thread is inserted to form a artery-vein Bypass,after 15 minutes,the blood flow was interrupted and the silk thread was taken out.The microelectronic balance was weighed.The wet weight and dry weight of the thrombus were compared between the groups.The abdominal aorta blood was centrifuged and serum was taken.The serum levels of thromboxane and6-keto-prostaglandin F1? were measured by ELISA.Observe the effect of "Glacier quick-acting rescue pills" on animal thrombosis.2.The rat model of acute blood stasis syndrome was replicated by subcutaneous injection of epinephrine hydrochloride and ice water.The blood was collected from the abdominal aorta.The blood rheometer was used to measure the whole blood viscosity at different shear rates.(WBV),plasma viscosity(PV),coagulometer was used to measure prothrombin time(PT)and thrombin time(TT)in each group of animals.Observe the effect of "Glacier quick-acting rescue heart pills" on blood rheology in rats.Results: 1."Bingchuansuxiaojiuxindiwan" on animal thrombosis research shows that:(1)Bingchuansuxiaojiuxindiwan high,medium and low dosage group and positive control group(Quick-acting Heart-saving Pills)thrombosis dry and wet weight are lower than model group thrombosis(P < 0.05,P < 0.001).(2)The determination of serum thromboxane(TXB2)showed that there was significant difference between the high dose group and the model group(P < 0.05).(3)The content of 6-keto-prostaglandin F1 alpha(6-k-PGF1 alpha)in positive control group and Bingchuansuxiaojiuxindiwan in middle and low dosage groups was higher than that in model group(P < 0.05,P < 0.01).(4)The ratio of 6-k-PGF1a/TXB2 decreased.2."Bingchuansuxiaojiuxindiwan" on the hemorheology of rats.(1)The whole blood viscosity and plasma viscosity of the high dose Bingchuansuxiaojiuxindiwan group were lower than those of the model group at different shear rates(P < 0.05).(2)The results of plasma coagulation factor test showed that the levels of TT and PT in high-dose animals of Bingchuansuxiaojiuxindiwan were significantly different from those of model group(P <0.05,P < 0.001).Conclusion: The experimental study on the effect of Bingchuansuxiaojiuixindiwan on activating blood circulation and removing blood stasis shows that Bingchuansuxiaojiuxindiwan can obviously inhibit the formation of arteriovenous bypass thrombosis in animals,and can reduce the whole blood viscosity and plasma viscosity of model animals at different shear rates.At the same time,it can reduce the content of plasma thromboxane(TXB2),increase the content of 6-keto-prostaglandin F1 alpha(6-k-PGF1alpha),reduce the ratio of them,and prolong the prothrombin time(PT)and 6-thrombin time(TT)in different degrees.It is suggested that the mechanism of activating blood circulation and removing blood stasis may be related to regulating the ratio of TXB2 to6-k-PGF1 alpha,prolonging prothrombin time(PT)and 6-thrombin time(TT),thus inhibiting platelet aggregation,reducing blood viscosity and inhibiting thrombosis.