Study On Expression Of Chemokine MIP-1α And Its Receptor CCR5 In Endometriosis

Objective:To study the expression of MIP-1α and CCR5 in eutopic endometrium,ectopic endometrium,peritoneal fluid and serum in endometriosis(EMT),and explore their clinical significance in EMT.Methods:Forty-five patients who underwent laparoscopy / laparotomy and pathologically confirmed as EMT were selected as the experimental group.Thirty patients who underwent laparoscopic / laparotomy and confirmed no pelvic EMT due to benign gynecological diseases were selected as the control group.The expression levels of MIP-1α and CCR5 in the ectopic endometrium,eutopic endometrium and normal endometrium were detected by immunohistochemistry.The relative expression levels of MIP-1α and CCR5 mRNA in the ectopic endometrium,eutopic endometrium and normal endometrium were detected by Real-time PCR.The expression levels of MIP-1α and CCR5 in the peritoneal fluid and serum were measured by ELISA.Results:(1)Immunohistochemical results: Compare to the control group,the expressions of MIP-1α and CCR5 at ectopic endometrium and eutopic endometrium in experimental group were higher(P<0.05),and the expression of them in ectopic endometrium was higher than in eutopic endometrium(P<0.05).And in the experimental group,the expressions of MIP-1α and CCR5 in the patients with stage III-IV were higher than thepatients with stage I-II(P<0.05).There were positive correlations between the relative expression of MIP-1α and CCR5 both at ectopic endometrium and eutopic endometrium in the experimental group.(2)Real-time PCR results: Compare to the control group,the relative expressions of MIP-1α and CCR5 mRNA at ectopic endometrium and eutopic endometrium in experimental group were higher(P<0.05),and the relative expression of them in ectopic endometrium was higher than in eutopic endometrium(P<0.05).And in the experimental group,the relative expressions of MIP-1α and CCR5 mRNA in the patients with stage III-IV were higher than the patients with stage I-II(P<0.05).There were positive correlations between the relative expression of MIP-1α and CCR5 mRNA both at ectopic endometrium and eutopic endometrium in the experimental group.(3)Enzyme-linked immunosorbent assay results: The expressions of MIP-1α and CCR5 in the peritoneal fluid of the experimental group were higher than those in the control group(P <0.05),and the expression level of MIP-1α and CCR5 in the peritoneal fluid of the experimental group was positively correlated with plevic pain.Compare to the control group,the expressions of MIP-1α and CCR5 in the serum of the experimental group were not statistically significant(P> 0.05).Conclusion:(1)Compare to the control group,MIP-1α and CCR5 are highly expressed at ectopic endometrium,eutopic endometrium and peritoneal fluid in experimental group,indicating that MIP-1α and CCR5 play the important role in the occurrence and development of EMT.(2)The contents of MIP-1α and CCR5 of the peritoneal fluid in the experimental group were higher than those in the control group,and there was a certain correlation with the degree of plevic pain in the experimental group,indicating that both of them are involved in the change of the intra-abdominal environment of EMT,which may explain the EMT ectopic focal adhesions,implantation and blood vessel formation,and providethe evidence for chronic pelvic pain.(3)There was no significant difference in the contents of MIP-1α and CCR5 in serum between the experimental group and the control group.(4)The expressions of MIP-1α and CCR5 at ectopic endometrium,eutopic endometrium and peritoneal fluid in experimental group are correlated,indicating that the two factors interact with each other and affect the development of EMT.