Correlations Between 3.0T MR Features And Molecular Marker Ki67、P504s In Prostate Cancer

Part Ⅰ Correlation Between Parameters of Diffusion Weighted Imaging,Dynamic Contra st-enhanced Magnetic Resonance Imaging and the Expression of Ki67 Protein in Prostate CancerObjective:To investigate the correlation between diffusion-weighted imaging（DWI）and dynamic contrast-enhanced magnetic resonance imaging（DCE-MRI）parameters of prostate cancer and Ki67 protein expression.Methods:A total of 93 patients with prostate disease（including prostate cancer 、 benign prostatic hyperplasia and/or chronic prostatitis）confirmed by pathology were enrolled in the study.Multi-modality magnetic resonance imaging（mp MRI）was performed to obtain the time-signal intensity（SI-T）curve and peak time（Tmax）of the prostate cancer group and non-prostate cancer group.Maximum enhancement SI（SImax）,fastest enhancement rate（Rmax）and automatically generate apparent diffusion coefficient（ADC）values were obtained;immunohistochemical was used to detect the expression of Ki67 and its correlation with MRI parameters.Result:The ADC values,Tmax,SImax and Rmax of the prostate cancer group and the non-prostate cancer group were（0.76±0.16）x10^-3 mm²/s,（18.61±4.33）s,（1.74±0.39）%,（21.03±14.72）%,respectively;and（1.36±0.19）x10^-3mm²/s,（37.45±19.51）s,（1.32±0.45）%,（10.11±6.22）%,the dif ference between the two groups was statistically significant（P < 0.001）.The ADC values,Tmax,SImax and Rmax of the Ki67 negative group were（1.26±0.25）x10^-3mm²/s,（34.44 ±20.19）s,（1.37±0.47）%,（9.92±8.87）%,respectively;Ki67 low expression group were（0.95±0.36）x10^-3mm²/s,（23.74±12.44）s,（1.62±0.40）%,（19.28±12.41）%;Ki67 high expression group were（0.70±0.06）x10^-3mm²/s,（18.87±4.74）s,（1.85±0.45）%,（23.64±19.51）%.For Ki67 negative group,Ki67 low expression group and Ki67 high expression group,there were significant differences between these groups（H= 32.588,18.719,10.867,18.595;P < 0.05）.However,there was no significant difference between the Ki67 low expression group and the Ki67 high expression group（P > 0.05）.ADC values and Tmax were negatively correlated with Ki67（r =-0.50 and-0.37,P < 0.01）;SImax and Rmax were positively correlated with Ki67（r = 0.29 and 0.36,P < 0.01）.Conclusion:The ADC value of prostate cancer and DCE-MRI perfusion index are correlated with Ki67.The biological characteristics of prostate cancer can be evaluated non-invasively,and it can be used as an imaging biological indicator to predict the degree of tumor malignancy.Part Ⅱ Multi-modality MRI parameters correlate with the expression of the P504 s protein in prostate cancerObjective:To evaluate the expressions of P504 s,34β-E12,and P63 protein in PCa and the correlation between the parameters of multi-modality MRI and the expression of P504 s.Methods:We retrospectively analyzed the multi-modality MRI data on 43 PCa and 64 nonPCa patients.We obtained the signal intensity-time（SI-T）curve,maximum SI（SImax%）,time to SImax（Tmax）,rate of maximum enhancement（Rmax）,and automatically generated apparent diffusion coefficient（ADC）values by conventional,diffusion-weighted and dynamic contrast-enhanced MRI,and determined the expressions of P504 s,34β-E12 and P63 in the prostatic tissues of the patients by immunohistochemistry.Result:Statistically significant differences were observed between the PCa and non-PCa groups in the positive expressions of P504s（83.7% vs 0%,P < 0.05）,34β-E12（25.6% vs 91.0%,P< 0.05）and P63（25.6% vs 86.0%,P < 0.05）in the prostatic tissues,the ADC value([0.83±0.22]vs [1.34±0.28]x10^-3mm²/s,P < 0.05),Tmax（[21.30±10.78] vs [50.22±36.31] s,P < 0.05）,SImax（[1.75±0.39]% vs [1.24±0.41]%,P < 0.05）,and Rmax（[20.20±15.50]% vs [7.98±6.25]%,P <0.05）.The expression of P504 s was correlated negatively with the ADC value and Tmax（r =-0.60 and-0.37,P < 0.01）but positively with SImax and Rmax（r = 0.50 and 0.45,P < 0.01）.Conclusion:The parameters of multi-modality MRI are correlated with the expression of P504 s in PCa and can be used as imaging biomarkers for predicting the degrees of its malignancy.