The Investigation Of ARID1A And HIF-1A Expression And Correlation In Gastric Cancer

Gastric cancer(GC)is one of the most common malignant tumors,with high morbidity and mortality.Surgical treatment,including chemotherapy,has developed rapidly in recent years.However,the five-year survival rate of GC patients remains very low.Even accepted the standard treatment for GC,a lot of patients suffered recurrence,drug resistance and side effects,which seriously affect the prognosis and quality of life of patients.Therefore,further explore the underlying mechanism of malignant tumors(including gastric cancer)might provide experimental basis for diagnosis,treatment and prognosis.Hypoxia is one of the most important characteristics of tumor microenvironment.Under the condition of hypoxia,HIF-1α can’t be degraded by ubiquification,and translocated into nuclear,and therefore play an essential role on processes of tumor energy metabolism,angiogenesis,and regulate cell cycle to promote tumor growth and metastasis by regulating its downstream protein expression.ARID1A is a member of the SWI/SNF family of chromatin remodeling complexes.In recent years,ARID1 A has been identified as an important tumor suppressor gene.Absent or low expression of ARID1 A in malignant tumors such as gastric cancer,is closely correlated with poor prognosis and metastasis of patients.However,the role of ARID1 A in GC under the condition of hypoxia had never been evaluated.Therefore,the current study analyzes the mRNA and protein expression of ARID1 A and HIF-1A in gastric cancer tissues and normal gastric tissues through searching databases(such as GEIPA and Oncomine)and IHC analysis.The correlation between mRNA or protein expression of ARID1 A and HIF-1A with survival,clinical characteristics and their expression in gastric carcinoma tissue was analyzed.The current study revealed that the expression of ARID1 A mRNA in gastric carcinoma tissue did not change significantly,and its low expression ARID1 A mRNA significantly correlated with the poor prognosis of patients.The protein expression of ARID1 A was significantly decreased in gastric carcinoma tissue,while there was no significant correlation with the clinical characteristics such as age and tumor size of GC patients.The both mRNA and protein expression of HIF-1A in gastric cancer tissues was significantly increased,and the higher mRNA expression of HIF-1A was obviously correlated with poor prognosis of GC patients,but there was no significant correlation with the clinical characteristics such as age and tumor size of GC patients.Moreover,the low expression of ARID1 A was significantly related to the high expression of HIF-1A in gastric cancer tissue.Based on the above studies,the results show that the low expression of ARID1 A in gastric cancer tissue is significantly correlated with high expression of HIF-1A in gastric cancer tissue,suggesting that ARID1 A may be involved in the regulation of hypoxic microenvironment mediated by HIF-1A.This experiment provides a basis for further research on the regulatory effects of ARID1 A and HIF-1A and its regulatory effects on tumor microenvironment.